中国药房2017，Vol.28Issue(34)：4788-4792,5.DOI:10.6039/j.issn.1001-0408.2017.34.09
当归芍药散对围绝经期模型大鼠子宫结构及雌激素受体表达的影响
Effect of Danggui Shaoyao Powder on the Uterine Structure and Estrogen Receptor Expression in Model Rats in Perimenopausal Period
摘要
Abstract
OBJECTIVE:To investigate the effect of Danggui shaoyao powder (DSS) on uterine structure and expressions of estrogen receptorα(ERα),estrogen receptorβ(ERβ)in uterine cavity epithelium and matrix in model rats in perimenopausal peri-od. METHODS:40 female SD rats were randomly divided into sham operation group (normal saline),model group (normal sa-line),DSS low-dose,medium-dose,high-dose groups(1.94,3.87,7.44 g/kg),8 in each group. Except that rats in sham opera-tion group received resection of fat nearby ovaries,rats in other groups received resection of bilateral ovaries to induce models in perimenopausal period. After modeling,rats were intragastrically administrated once a day,for 8 weeks. After administration,wet mass of uterine was weighted. Changes in uterine morphology and structure of rats were observed,expressions levels of ERα and ERβ in uterine cavity epithelium and matrix were determined. RESULTS:Compared with sham operation group,rats in model group showed low columnar in endometrial columnar epithelium,lamina propria layer,muscular layer and serous layer were signifi-cantly atrophied,stromal cells had obvious nuclear condensation. There was marked decrease in uterine wet mass,uterine cavity ar-ea and endometrial thickness as well as the number of uterine glands(P<0.01),and the expression levels of ERαand ERβin uter-ine cavity epithelium and matrix were significantly reduced(P<0.01). Compared with model group,the atrophy degree of endome-trium and lamina propria layer had no significant differences in DSS each dose groups. However,lamina propria layer was rich in glands,and there were significant differences in uterine wet mass,uterine cavity area,endometrial thickness,ERα expression level in uterine cavity epithelium and matrix (P>0.05). However, the number of uterine glands in DSS medium-dose,high-dose groups was significantly increased(P<0.01),and ERβexpres-sion level in uterine cavity epithelium and matrix in DSS high-dose group was significantly increased (P<0.05 or P<0.01). CONCLUSIONS:DSS has not obvious effect on im-proving the symptoms of uterine gland atrophy of model rats in perimenopausal period,but it can increase the number ofuterine glands,and the mechanism may be associated with improving the ERβ expression level in uterine cavity epithelium and ma-trix.关键词
当归芍药散/围绝经期/子宫结构/雌激素受体α/雌激素受体β/大鼠Key words
Danggui shaoyao powder/Perimenopausal period/Uterine structure/Estrogen receptor α/Estrogen receptor β/Rats分类
医药卫生引用本文复制引用
丁岩,华永庆,林紫微,杨菲,洪敏..当归芍药散对围绝经期模型大鼠子宫结构及雌激素受体表达的影响[J].中国药房,2017,28(34):4788-4792,5.基金项目
国家自然科学基金资助项目(No.81473390) （No.81473390）
江苏省中药资源产业化过程协同创新中心开放课题(No.ZDXM-2-7) （No.ZDXM-2-7）
江苏省中药药效与安全性评价重点实验室资助项目(No.JKLPSE201607) （No.JKLPSE201607）
江苏省高校中药学优势学科建设工程资助项目(No.PAPD) （No.PAPD）
