中国药房2017，Vol.28Issue(34)：4811-4814,4.DOI:10.6039/j.issn.1001-0408.2017.34.15
葡萄籽原花青素对肾缺血再灌注损伤大鼠肾组织中Caspase-3和TRAF6表达的影响
Effect of Grape Seed Procyanidins on Protein Expressions of Caspase-3 and TRAF6 in Renal Tissue of Rats with Renal Ischemia-reperfusion Injury
摘要
Abstract
OBJECTIVE:To study the effect of grape seed procyanidins on protein expressions of cysteine aspartic protease 3 (Caspase-3), tumor necrosis factor-related receptors (TRAF6) in renal tissue of rats with renal ischemia-reperfusion injury (RIRI),and explore the protective mechanism of procyanidins on RIRI. METHODS:50 rats were randomly divided into sham op-eration group,model group,Shenfukang capsules group(positive control,600 mg/kg),procyanidins low-dose,high-dose groups (100,150 mg/kg),10 in each group. All rats were intragastrically administrated once a day,for 7 d. After administration,rats in other groups except for sham operation group were established the RIRI model.After the modeling successed of 24 h,levels of creati-nine(Cr),urea nitrogen(BUN)in serum,and protein expressions of Caspase-3,TRAF6 in renal tissue of rats in each group were detected,and apoptotic rate of renal tubular epithelial cells was determined. RESULTS:Compared with sham operation group,lev-els of Cr,BUN in serum in model group were significantly increased(P<0.05);protein expressions of Caspase-3,TRAF6 in re-nal tissue were obviously enhanced (P<0.05);apoptotic rate of renal tubular epithelial cells was obviously increased (P<0.05). Compared with model group,levels of Cr,BUN in serum in each administration group were obviously decreased(P<0.05);pro-tein expressions of Caspase-3,TRAF6 in renal tissue were obviously weakened(P<0.05);apoptotic rate of renal tubular epithelial cells was obviously decreased(P<0.05);and procyanidins high-dose group showed superior effect to low-dose group and Shenfu-kang granules group (P<0.05). CONCLUSIONS:Grape seed procyanidins can relieve the RIRI of rats,which may be achieved by reducing protein expressions of Caspase-3 and TRAF6 to inhibit the apoptotic rate of renal tubular epithelial cells.关键词
葡萄籽原花青素/肾缺血再灌注损伤/半胱氨酸天冬氨酸蛋白酶3/肿瘤坏死因子相关受体/大鼠Key words
Grape seed procyanidins/Renal ischemia-reperfusion injury/Cysteine aspartic protease 3/Tumor necrosis fac-tor-related receptors/Rats分类
医药卫生引用本文复制引用
宋云梅..葡萄籽原花青素对肾缺血再灌注损伤大鼠肾组织中Caspase-3和TRAF6表达的影响[J].中国药房,2017,28(34):4811-4814,4.基金项目
南阳市科技攻关项目(No.2014GG040) （No.2014GG040）
