中国药房2017，Vol.28Issue(34)：4830-4834,5.DOI:10.6039/j.issn.1001-0408.2017.34.20
维药毛菊苣95%乙醇提取物对刀豆蛋白A诱导小鼠免疫性肝损伤的保护作用及机制研究
Study on the Protective Effect and Mechanism of Wei Medicine Cichorium glandulosum 95% Ethanol Ex-tract on Con-A-induced Immunological Liver Injury in Mice
摘要
Abstract
OBJECTIVE:To investigate the protective effect and mechanism of Wei medicine Cichorium glandulosum 95% eth-anol extract(CG-I)on immunological liver injury in mice,and provide reference for post-screening its effective site. METHODS:60 mice were randomly divided into blank control group(normal saline),model group(normal saline),positive control group(Di-ammonium glycyrrhizinate,100 mg/kg) and CG-I high-dose,medium-dose,low-dose groups (calculated by crude drugs as 200, 100,50 g/kg),10 in each group,intragastrically administrated once every day,for 10 d. After 1 h of last administration,except for blank control group,mice in other groups were intravenously injected Con-A in tail to induce immunological liver injury. After 8 h of modeling,tumor necrosis factor α(TNF-α),interferon γ(IFN-γ),interleukin 1β(IL-1β)contents in serum were detected;liver and spleen indexes were calculated. The pathological changes in liver tissue were observed,and aspartate aminotransferase(AST), alanine aminotransferase (ALT), glutathione S-transferase (GST), alkaline phosphatase (AKP), total superoxide dismutase (T-SOD),malondialdehyde(MDA)levels in liver tissue were detected. RESULTS:Compared with blank control group,TNF-α, IFN-γ,IL-1β contents in serum in model group were significantly increased;liver,spleen indexes and AST,ALT,GST,AKP, T-SOD levels in liver tissue were significantly increased;and MDA level in liver tissue was significantly reduced,with statistical significances(P<0.05 or P<0.01);liver of mice in model group was cluttered,showing swelling,necrosis and other diseases in liver cells. Compared with model group, except that AST, ALT,AKP levels in liver tissue in CG-I low-dose group and MDA, IFN-γ contents in serum in CG-I medium-dose, low-dose groups had no significant decrease, other indexes were significantly improved (P<0.05 or P<0.01);and patho-logical changes in liver tissue were relieved to varying degrees. CONCLUSIONS:CG-I shows protective effect on Con-A-in-duced immunological liver injury in mice,especially the high dose and medium dose. The mechanism may be associated with its an-ti-oxidation and anti-inflammatory effects.关键词
维药/毛菊苣/95%乙醇提取物/免疫性肝损伤/抗氧化/抗炎/小鼠Key words
Wei medicine/Cichorium glandulosum/95% ethanol extract/Immunological liver injury/Anti-oxidant/Anti-in-flammatory/Mice分类
医药卫生引用本文复制引用
胡利萍,张钰,秦冬梅,依巴古力·艾拜都拉,努力比亚·阿布都克尤木,孙晓风,张跃新..维药毛菊苣95%乙醇提取物对刀豆蛋白A诱导小鼠免疫性肝损伤的保护作用及机制研究[J].中国药房,2017,28(34):4830-4834,5.基金项目
新疆维吾尔自治区自然科学基金资助项目(No.2015211C048) （No.2015211C048）
