中国药房2017，Vol.28Issue(34)：4856-4859,4.DOI:10.6039/j.issn.1001-0408.2017.34.27
多指标-正交试验优化沙棘叶和沙棘籽中黄酮的提取工艺
Optimization of Extraction Technology for Flavonoids in Leaves and Seeds of Hippophae rhamnoides by Multiindex-Orthogonal Test
摘要
Abstract
OBJECTIVE:To optimize the extraction technology for flavonoids in leaves and seeds of Hippophae rhamnoides. METHODS:Using the total extraction rate of 6 flavonoid aglycones(catechins,rutin,myricetin,quercetin,kaempferol,isorham-netin)as index,ethanol volume fraction,extraction time,extraction times,material-lipid ratio as investigation indexes,L9(34)or-thogonal test was designed to optimize the extraction technology of flavonoids in leaves and seeds of H. rhamnoides,and verifica-tion test was carried out. RESULTS:The optimum extraction technology for flavonoids in leaves of H. rhamnoides was ethanol vol-ume fraction of 70%,extracting for 3 times with material-lipid ratio of 1:16,and 2.0 h each time;and that of seeds was ethanol volume fraction of 50%,extracting for 3 times with material-lipid ratio of 1:24,and 1.5 h each time. In verification test,the total extraction rate of 6 flavonoid aglycones was 56.4 mg/g in the leaves (RSD=1.4%,n=3) and 15.4 mg/g in the seeds (RSD=3.4%,n=3). CONCLUSIONS:Optimized extraction technology is simple,stable,feasible,and can be used for extracting the fla-vonoids in leaves and seeds of H. rhamnoides.关键词
沙棘叶/沙棘籽/正交试验/提取工艺/黄酮苷元Key words
Leaves of Hippophae rhamnoides/Seeds of Hippophae rhamnoides/Orthogonal test/Extraction technology/Fla-vonoid aglycone分类
医药卫生引用本文复制引用
惠人杰,冯静,蔺默含,冯柏年..多指标-正交试验优化沙棘叶和沙棘籽中黄酮的提取工艺[J].中国药房,2017,28(34):4856-4859,4.基金项目
江苏省自然科学基金青年基金项目(No.BK20140136) （No.BK20140136）
江苏高校品牌专业建设工程资助项目(No.PPZY2015B146) （No.PPZY2015B146）
