中国药房2017，Vol.28Issue(34)：4868-4871,4.DOI:10.6039/j.issn.1001-0408.2017.34.30
天冬总皂苷的大孔树脂纯化工艺研究
Study on the Purification Technology of Total Saponins from Asparagus cochinchinensis with Macroreticu-lar Resin
摘要
Abstract
OBJECTIVE:To optimize the purification technology of total saponins from Asparagus cochinchinensis with macro-reticular resin. METHODS:Using content of total saponins from A. cochinchinensis as index,single factor test was used to investi-gate the macroreticular resin model,sampling adsorption time,mass concentration of the column,adsorption capacity,volume frac-tion and the amount of elution solvent,elution rate,and optimize the purification technology. And verification test was conducted. RESULTS:HPD-300 macroreticular resin showed strong absorption and desorption property. The optimal purification technology was that sampling adsorption time was 60 min,mass concentration of sample liquid was 0.1 g/mL,adsorption capacity was 120 mL(15 BV),it was eluded with 60% ethanol solution with 3 BV and elution rate was 4 BV/h. In the verification test,the average desorption rate of total saponins was 68.30%(RSD=0.95%,n=3). CONCLUSIONS:Optimized purification technology is sta-ble,feasible,and can easily separate and purify the total saponins from A. cochinchinensis.关键词
天冬/总皂苷/大孔树脂/纯化工艺Key words
Asparagus cochinchinensis/Total saponins/Macroreticular resin/Purification technology分类
医药卫生引用本文复制引用
刘亮,杜江,冯华,丁丽娜,陈东林,周英信..天冬总皂苷的大孔树脂纯化工艺研究[J].中国药房,2017,28(34):4868-4871,4.基金项目
遵义市科技计划课题(No.遵市科合社字〔2013〕24号) （No.遵市科合社字〔2013〕24号）
