中国肿瘤生物治疗杂志2017，Vol.24Issue(11)：1260-1265,6.DOI:10.3872/j.issn.1007-385x.2017.11.003
沉默Gli2基因对肝癌SMMC-7721细胞增殖和凋亡的影响
Effect of silencing Gli2 gene on proliferation and apoptosis of the hepatocellular carcinoma SMMC-7721 line cell
摘要
Abstract
Objective:To explore effect of silencing glioma associated oncogene homolog 2 (Gli2) on proliferation and apoptosis of the liver cancer SMMC-7721 cell and its possible action mechanism.Methods:Infection of the SMMC-7721 cell with recombinant shRNA-Gli2 lentivirus was used to screen the best jamming sequence for silence effect.The SMMC-7721 cell of interference group was infected with the best jamming sequence lentivirus,the SMMC-7721 cell of control group was infected with shRNA-NC shRNA-Gli2 lentivirus and the SMMC-7721 cell of blank group didn't with any treatment.CCK-8 and Western blotting assays were used to detect proliferation activity of the SMMC-7721 cell in various groups.Flow cytometry assay was used to test apoptosis of the SMMC-7721 cell in various groups.Expressions of Cli2,cyclinD1,Bax and Bcl-2 mRNA and their proteins were detected by qPCR and Western blotting.Results:Infection rate of the recombinant lentivirus was about 90％.Silence effect of the recombinant shRNA-Gli2-1 lentivirus was the best.Expressions of Gli2 mRNA and its protein in the SMMC-7721 cell of interference group were remarkably lower than those in the SMMC-7721 cells of control and blank groups (all P＜0.05),and differences of Gli2 mRNA and its protein expressions between the SMMC-7721 cells of control group and blank group were not significant (P＞0.05).Proliferation and cell colony forming number of the SSMC-7721 cell in interference group were obviously less than those of the SSMC-7721 cell in control and blank groups (all P＜0.05).Apoptosis rate of the SMMC-7721 cell in interference group evidently higher than those of the SMMC-7721 cell in control and blank groups (P＜0.01).Expressions of cyclinD1 and Bax mRNA and their proteins in the SMMC-7721 cell of interference group were observably lower than those in the SMMC-7721 cells of control and blank groups (all P＜0.05),but expressions of Bcl-2 mRNA and protein in the SMMC-7721 cell of interference group were observably higher than those in the SMMC-7721 cells of control and blank groups (all P＜0.05).Conclusion:Silencing expression of Gli2 gene could inhibit growth of the liver cancer SMMC-7721 cell,and promote apoptosis of the liver cancer cell,its mechanism might relate to down-regulation of cyclin D1 and Bax as well as upregulation of Bcl-2.关键词
胶质瘤相关癌基因同源蛋白2基因/肝癌/细胞增殖/细胞凋亡Key words
glioma associated oncogene homolog 2 gene (Gli2)/liver cancer/cell proliferation/cell apoptosis分类
医药卫生引用本文复制引用
石刚,吴斌,严舒,张光年,兰川,曾丽娟,李建水..沉默Gli2基因对肝癌SMMC-7721细胞增殖和凋亡的影响[J].中国肿瘤生物治疗杂志,2017,24(11):1260-1265,6.基金项目
四川省卫生计生委科技科研项目资助(No.17PJ590). Project supported by the Scientific Research Foundation of the Health and Family Planning Commission of Sichuan Province (No.17PJ590) （No.17PJ590）
