中国肿瘤生物治疗杂志2017，Vol.24Issue(11)：1266-1270,5.DOI:10.3872/j.issn.1007-385x.2017.11.004
IL-21增强CIK细胞对食管癌EC9706细胞的杀伤作用及其可能的机制
IL-21 enhances the activity of CIK killing the esophageal cancer EC9706 cell and its possible mechanism
摘要
Abstract
Objective:To explore effect of cytokine interleukin 21 (IL-21) on the activity of cytokine-induced killer (CIK) cell killing in vitro the esophageal cancer EC9706 cell,and preliminarily to analyze its possible molicular mechanism.Methods:Human peripheral blood mononuclear cells were isolated with sterile operation in vitro,which were respectively cultured into CIK cells in routine medium or in routine medium plus IL-21.Flow cytometry assay was used to detect immunophenotypes of CIK cells cultured with the two medium.Lactate dehydrogenase (LDH) release method was used to exam the activities of the two cultured CIK cells killing the esophageal cancer EC9706 cell.Concentrations of IFN-γ in liquid supematant cultured the two types of CIK cell were checked by ELISA.Results:There not were any significant difference of proliferation number of the CIK cells between routine culture and routine culture plus IL-21.Proportion of CD3+CD56+ cell,expression rates of granzyme B and perforin in CD3+ cell of the CIK cell cultured by routine medium plus IL-21 were evidently higher than those of the CIK cell cultured by routine medium (CD3+CD56+cell:[26.95±3.53]％ vs [16.18± 1.04]％;granzyme B:[33.29±2.30]％ vs [23.58±2.28]％;perforin:[59.70±1.91]％ vs [45.96±2.67]％,all P＜0.05).At effector/target ratio of 20∶1 and 30∶ 1,killing activities against the EC9706 cell of the CIK cell cultured with routine medium plus IL-21 were all remarkably higher than those of the CIK cell cultured with routine medium (at E/T 20∶1 [43.66±1.99]％ vs [34.59±1.75]％;at E/T 30∶1 [142.7± 13.4]％ vs [42.6±3.3]％,all P＜0.05).Concentration of IFN-γ in liquid supernatant cultured the CIK cell in routine medium plus IL-21 was obviously higher than that cultured the CIK cell in routine medium [(157.2± 10.3) ng/L vs (46.2±4.3) ng/L,P＜0.05].Conclusion:IL-21 could enhance killing activity of the CIK cell against the esophageal cancer EC9706 cell through increasing proportion of CD3+CD56+ cell,expression rates of granzyme B and perforin in CD3+ cell as well as raising the secretion of IFN-γ by the CIK cell.关键词
食管癌/细胞因子诱导的杀伤细胞/白介素-21/穿孔素/颗粒酶B/EC9706细胞Key words
esophageal cancer/cytokine-induced killer (CIK) cell/interleukin 21 (IL-21)/perforin/granzyme B/EC7906 cell分类
医药卫生引用本文复制引用
肖鹏,冯睿婷,梅家转,赵继智..IL-21增强CIK细胞对食管癌EC9706细胞的杀伤作用及其可能的机制[J].中国肿瘤生物治疗杂志,2017,24(11):1266-1270,5.基金项目
河南省卫生厅科技攻关项目资助(No.201303222).This project was supported by the Foundation of Henan Provincial Health Bureau for Scientific and Technological Program (No.201303222) （No.201303222）
