中国肿瘤生物治疗杂志2017，Vol.24Issue(11)：1271-1275,5.DOI:10.3872/j.issn.1007-385x.2017.11.005
HuR siRNA对肺腺癌A549细胞增殖、迁移及侵袭的影响
Effects of HuR siRNA on proliferation, migration and invasion of the lung adenocarcinoma A549 cells
摘要
Abstract
Objective:To explore effect of HuR siRNA on proliferation,migration and invasion of the human lung adenocarcinoma cell line A549 and its possible mechanism.Methods:After the A549 cells were transfected by HuR siRNA and cultured for 24 hours,CCK-8 assay and clone formation assay were used to detect proliferation ability of the A549 cells.Migration and invasion abilities of the A549 cells were respectively detected by scratch and Transwell assays.mRNA and protein expressions of HuR and matrix metalloproteinose-9 (MMP-9) were detected by RTqPCR and Westem blotting assays.Results:HuR siRNA inhibited proliferation,migration and invasion of the human lung cancer cell line A549.HuR siRNA significantly reduced mRNA and protein expressions of HuR and MMP-9 in the A549 cells (P＜0.01).Conclusion:Down-regulation of HuR could inhibit proliferation,migration and invasion of the lung adenocarcinoma A549 cells,and the inhibitions of migration and invasion of the A549 cells might be related to down-regulation of MMP-9.关键词
人抗原R/肺腺癌/肺腺癌A549细胞/基质金属蛋白酶-9/侵袭/迁移Key words
human antigen R (HuR)/lung adenocarcinoma/lung adenocarcinoma/A549 cells/MMP-9/invasion/migration分类
医药卫生引用本文复制引用
申庆鹏,王俊,王宝成..HuR siRNA对肺腺癌A549细胞增殖、迁移及侵袭的影响[J].中国肿瘤生物治疗杂志,2017,24(11):1271-1275,5.基金项目
国家自然科学基金资助项目(No.81572875,81272619).Project supported by the National Natural Science Foundation of China (No.81572875,81272619) （No.81572875,81272619）
