中国肿瘤生物治疗杂志2017，Vol.24Issue(11)：1276-1280,5.DOI:10.3872/j.issn.1007-385x.2017.11.006
丹参酮ⅡA磺酸钠通过降低整合素β3的表达抑制宫颈癌HeLa细胞迁移
Sodium tanshinone ⅡA sulfonate inhibits migration of the cervix carcinoma HeLa cell via down-regulating expression of integrin β3
李军峰 1沈净净 2张冬冬 1张英姿3
作者信息
- 1. 枣庄矿业集团枣庄医院肿瘤科,山东枣庄 277101
- 2. 枣庄市薛城区人民医院妇产二科,山东枣庄 277000
- 3. 滨州医学院附属医院妇科,山东滨州 256603
- 折叠
摘要
Abstract
Objective:To explore effect of sodium tanshinone ⅡA sulfonate (STS) on migration of the cervix carcinoma Hela cell and its possible mechanism.Methods:Effects of STS in different concentrations,specific antibody to integrin β3 (LM609),and transfection with integrin β3 over-expression plasmid on migration of the cervix carcinoma HeLa cell were detected by Transwell assay.Immunoblotting test was used to detect effects of STS in different concentrations and different times of STS stimulation on expression of integrin β3 in the HeLa cell.Effect of STS on viability of the HeLa cell was tested by CCK-8 assay.Results:STS in concentration-dependent manner reduced significantly migration of the HeLa cell (P＜0.01).LM609 remarkably decreased migration rate of the HeLa cell (P＜0.01).Migration rate of the HeLa cell transfected with over-expression integrin β3 plasmid and stimulated by STS was markedly more increase than those of the HeLa cell stimulated by STS only as well as the HeLa cell transfected with empty plasmid and stimulated by STS (P＜0.01).STS in concentration-and timing-dependent manners reduced expression of integrin β3 in the HeLa cell (P＜0.05).There was no significant difference in number of the viable cells between the HeLa cell stimulated with different concentrations of STS groups and the HeLa cells in blank control group (P＞0.05).Conclusion:STS could reduce expression of integrin β3 in the cervix carcinoma HeLa cell,and significantly decrease migration of the HeLa cell,LM609 which is a specific antibody against integrin β3 could also reduced migration of the HeLa cells,and transfection with integrin β3 over-expression plasmid could reduce the inhibition of STS on migration of the HeLa cell.The results of this paper shown that STS might inhibits migration of the HeLa cell via decrease of integrin β3 expression,which might provide theoretical basis and experimental evidence for further explain molecular mechanism of STS anti-carcinoma and clinical application of STS.关键词
丹参酮ⅡA磺酸钠/宫颈癌/HeLa细胞/整合素β3Key words
sodium tanshinone ⅡA sulfonate (STS)/cervix carcinoma/HeLa cell/integrin β3分类
医药卫生引用本文复制引用
李军峰,沈净净,张冬冬,张英姿..丹参酮ⅡA磺酸钠通过降低整合素β3的表达抑制宫颈癌HeLa细胞迁移[J].中国肿瘤生物治疗杂志,2017,24(11):1276-1280,5.
