摘要
Abstract
Objective:To detect expression of human interleukin 11 (IL-11) gene in tissue and blood specimens of the patients with diffuse large B-cell lymphoma (DLBCL),and to explore the relationship between expression of IL-11 gene and prognosis of the patients with DLBCL as well as its effect on thrombocytopenia of the patients with DLBCL after chemotherapy.Methods:Tissue and blood specimens of 88 patients with DLBCL and 42 patients with reactive lymphoid hyperplasia (RLH) who were made a definite diagnosis in Department of Hematology,the First Hospital of Handan City of Hebei Province during January 2012 to December 2016 were collected,qPCR was used to detect expression of IL-11 in the tumor tissues and blood specimens.Univariate and multivariate Cox regression analyses were used to analyze relationship of IL-11 expression in the tissues with clinical pathological features and prognosis of the patients with DLBCL as well as independent factors effecting prognosis of the patients.Correlationship between expression of IL-11 and thrombocytopenia after chemotherapy in the patients with DLBCL was analyzed,as wall as relationships of the IL-11 expression with overall survival (OS) and progression free survival (PFS)were analyzed by Kaplan-Meier assay.Results:Comparing with the patients with RLH in control group,expressions of IL-11 in tissue and blood of the patients with DLBCL were significant higher (in tissue:7.002±0.357 vs 1.507±0.139;in blood:6.700±0.337 vs 1.446±0.126,all P＜0.01).In the patients with DLBCL,expression of IL-11 was correlated to tumor size,clinical staging,B cell symptom,sensitivity of chemotherapy and IPI index (all P＜0.01).OS and PFS of the patients with high expression of IL-11 were shorter than those of the patients with low expression of IL-11 (all P＜0.01).Expression of IL-11,sensitivity of chemotherapy and IPI index were independent factors affecting prognosis of the patients with DLBCL.In the patients with thrombocytopenia after chemotherapy,expression of IL-11 in serum and platelet count were negative correlated(r=-0.732,P＜0.01).Conclusion:IL-11 was highly expressed in tissue and blood of the patients with DLBCL.OS and PFS of the DLBCL patients with high expression of IL-11 were significantly shortened,in whom,platelet severely reduced after chemotherapy.IL-11 might be expected to become an independent biological marker which could judge prognosis of the patients with DLBCL.关键词
弥漫性大B细胞淋巴瘤/白介素11基因/预后/血小板减少Key words
diffuse large B-cell lymphoma (DLBCL)/interleukin 11 (IL-11) gene/prognosis/thrombocytopenia分类
医药卫生
