中国肿瘤生物治疗杂志2017，Vol.24Issue(11)：1293-1298,6.DOI:10.3872/j.issn.1007-385x.2017.11.009
骨膜蛋白在乳腺癌中的表达及其临床意义
Expression of periostin in the mammary cancer and its clinical significance
摘要
Abstract
Objective:To explore expression status of periostin (POSTN) in the mammary cancer,para-cancer and normal mammary tissues and its clinical significance.Methods:The mammary cancer,para-cancer and normal mammary tissues of 45 patients with mammary cancer,which were taken out by exairesis in Department of Galactophore,Third Affiliated Hospital of Kunming Medical University during May 2012 to December 2016.In addition,from the tissues fifteen amicrobic mammary cancer and normal mammary tissues were selected for primary cell culture.The primary cultured ceils were identified by HE staining and immunohistochemistry assays.Expression status of POSTN mRNA in the mammary cancer,para-cancer and normal mammary tissues as well as in the cancer associated fibroblast (CAF) and normal fibroblast (NF) were detected by real-time quality PCR (qPCR).Correlation analysis was used to analyze the relationship between expression level of POSTN in the mammary tissue and clinical pathological features of the patients with mammary cancer.Location of POSTN protein in the mammary cancer tissue was identified by HE staining and immunohistochemistry of the mammary cancer tissue chips.Results:Expression level of POSTN mRNA in the mammary cancer tissue was significantly higher than those in the para-cancer and normal mammary tissues (P＜0.05),expression level of POSTN mRNA was similar between CAF and NF (P＞0.05).POSTN protein mainly expressed in substances of the mammary cancer tissues and less expressed in the mesenchyma of the mammary cancer tissue.Expression level of POSTN mRNA in the mammary cancer tissue was negatively correlated to estrogen receptor (ER) of the patients with mammary cancer (P＜0.05).Conclusion:POSTN was mainly expressed in substances of the mammary cancer tissues,high expression of which in the mammary tissues could be related to occurrence and development of the mammary cancer.关键词
骨膜素蛋白/乳腺癌/表达定位/临床意义Key words
periostin (POSTN)/mammary cancer/location of expression/clinical significance分类
医药卫生引用本文复制引用
李萍,王熙才,刘洪璐,黄鹏,刘馨..骨膜蛋白在乳腺癌中的表达及其临床意义[J].中国肿瘤生物治疗杂志,2017,24(11):1293-1298,6.基金项目
国家自然科学基金资助项目(No.81460358),云南省科技厅—昆明医科大学应用基础研究联合专项资助[No.2017FE467(-189),2014FB066] （No.81460358）
云南省卫计委科技计划资助项目(No.2017NS201,2014NS023).Project supported by the National Natural Science Foundation (No.81460358),the Joint Special Program of Yunnan Province Department and Kunming Medical University for applied basic research [No.2017FE467(-189),2014FB066],and the Science and Technology Planning Project of Yunnan Provincial Health and Family Planning Commission (No.2017NS201,2014NS023) （No.2017NS201,2014NS023）
