中国肿瘤生物治疗杂志2017，Vol.24Issue(11)：1309-1314,6.DOI:10.3872/j.issn.1007-385x.2017.11.012
影响肝癌预后独立危险因子的信息学分析
Informatics analysis of independent risk factors effecting on prognosis of the patients with liver cancer
摘要
Abstract
Objective:To explore independent risk factors affecting prognosis of the patients with liver cancer and their correlative features to action target points of targeting drug for liver cancer (sorafenib) via survey and analysis of bio-information.Methods:Data of gene expression,survival time and survival status of the 79 patients with liver cancer who finally died were selected from the 248 patients with liver cancer in the TCGA data base,the died patients were divided into 2 groups according to medium survival time of 360 days as a dividing line.Differential genes were screened out among data of the gene expression in patients of the 2 groups by DEseq algorithm.Without considering other mingled factors and only considering a single factor of differential gene expression and its correlation with survival time of the patients,uni-variate analysis of the differential genes was made by Kaplan-Meier assay.Survival analysis of a matrix integrated with the differential gene expression obtained by the analysis,survival time and survival status of the patients was made by COX regression model,in order to look for independent risk factors affecting prognosis of the patients with liver cancer.Based on STRING data base,a protein interconnected network was constructed with the obtained differential genes and action target points of sorafenib,targeting drug for liver cancer,to seek relationship of the action target points of sorafenib with the differential genes.Results:Fifty two differential genes that could affect prognosis of the patients with liver cancer were obtained by preliminary screening of DEseq algorithm.Twenty six differential genes relating to survival time of the patients were obtained by Kaplan-Meier assay.Three differential genes (SQSTM1,ANXA10 and STMN1) were finally confirmed as independent risk factors affecting prognosis of the patients with liver cancer by COX analysis.The protein-protein interaction network revealed that the action target points of sorafenib,targetin drug for liver cancer,were closely related to multiple differential genes.ANXA10 involved in combine of calcium ion and combination of calcium dependent phospholipids.STMN1 and SQSTM1 played a important role in multiple signaling pathways.Conclusion:ANXA10,STMN1 and SQSTM1 could be independent risk factors affecting prognosis of the patients with liver cancer,which might be used as action target points of targeting drug developed or prediction index of prognosis of the patients in future.关键词
肝癌/独立性危险因子/ANXA10基因/STMN1基因/SQSTM1基因Key words
liver cancer/independent risk factor/ANXA10 gene/STMN1 gene/SQSTM1 gene分类
医药卫生引用本文复制引用
韩涛,栾玉婷,葛亚楠,王识宇,杨晓丹,刘璐,刘兆喆,刘永叶,郑振东..影响肝癌预后独立危险因子的信息学分析[J].中国肿瘤生物治疗杂志,2017,24(11):1309-1314,6.基金项目
辽宁省博士科研启动基金资助项目(No.201501022) （No.201501022）
军队医学科技青年培育计划资助项目(No.15QNP001).Project supported by the Initial Foundation for Doctoral Science Research in Liaoning Province (No.201501022),and the Foundation for Development Program of Medical Science and Technology for Youth in the PLA (No.15QNP001) （No.15QNP001）
