浙江医学2017，Vol.39Issue(19)：1633-1637,后插1,6.DOI:10.12056/j.issn.1006-2785.2017.39.19.2017-1744
脂肪间充质干细胞复合人工真皮修复大鼠深度烧伤创面的研究
Adipose-derived mesenchymal stem cells combined with PELNAC for repairing deep burn wound in rats
摘要
Abstract
Objective To assess the efficacy of adipose-derived mesenchymal stem cells (ADSCs) combined with artificial dermis (PELNAC（R）) for repairing deep burn wound in rats.Methods ADSCs were isolated from adult SPF grade green fluorescent protein-transgenic rats.The specific cell surface markers of ADSC were analyzed with flow cytometry,the differentiation of ADSCs to osteoblasts was induced and the multilineage differentiation potential was observed.The third-degree burn wound model was induced on the back of Spregue-Dawley (SD) rats.The model rats were randomly divided into two groups:rats in test group (n=20) received ADSCs + PELNAC(R) for wound repairing and those in control group (n=20) received saline + PELNAC(R).The maximum diameter of wound on d7,d14 and d21 after burns was measured,respectively.On the d21 the skin samples of burn area were taken to evaluate wound healing by frozen section.Results The ADSCs were successfully isolated,cultured,and identified.The ADSCs had the ability to proliferate continuously and showed characteristics of multi-potent mesenchymal stem cells.The results of flow cytometry showed that CD44,CD90 and CD29 were highly expressed in ADSCs,while the hematopoietic cell surface marker CD34 and leukocyte common antigen CD45 were lowly expressed.On the d14 after burns,the maximum diameter of the wound was significantly lower in test group than that in control group (P＜0.05).On d21 after burns,fluorescence microscope showed scattered fluorescence was distributed in ADSCs-transplanted wound tissue in test group,but not in control group.Conclusion The combination of ADSCs with artificial dermis (PELNAC(R)) can significantly accelerate wound healing,indicating that it might be an effective method for treating deep burn.关键词
深度烧伤/人工真皮/脂肪间充质干细胞Key words
Deep burn/Artificial dermis/ADSCs引用本文复制引用
范友芬,乐欣,晋国营,虞耀华..脂肪间充质干细胞复合人工真皮修复大鼠深度烧伤创面的研究[J].浙江医学,2017,39(19):1633-1637,后插1,6.基金项目
浙江省医药卫生科技计划(2013KYB234) （2013KYB234）
