浙江医学2017，Vol.39Issue(20)：1735-1737,1741,后插1,5.DOI:10.12056/j.issn.1006-2785.2017.39.20.2017-770
乌司他丁对坏死性小肠结肠炎新生大鼠小肠结肠的保护作用研究
Protective effects of ulinastatin on necrotic enterocolitis in neonatal rats
摘要
Abstract
Objective To investigate the effects of ulinastatin on necrotic enterocolitis (NEC) in neonatal rats.Methods Sixty Sprague-Dawley rats aged of 3 days were randomly divided into four groups with 15 in each group:control,NEC model,NEC+Ulinastatin,and Ulinastatin.When experiments completed the intestinal samples were harvested and stained with hematoxylin and eosin (HE),the samples were observed under light microscope and scored according to the method of Shiou et al.Immunofluorescence was used to detect beclin-1 and LC3A/B to evaluate the autophagy and apoptosis.The inflammatory factors TNF-α,IL-1 β,and IL-6 were determined by enzyme-linked immunosorbent assay.Results HE staining revealed that the inflammatory response in NEC+Ulinastatin group was significantly attenuated compared with that in the NEC model group.The pathological examination showed a significant difference in Shiou scores among 4 groups.The expression of beclin-1 and LC3A/B levels in intestinal tissue of NEC+Ulinastatin group were significantly decreased;and the levels of inflammatory factors TNF-α,IL-1 β,and IL-6 were also significantly decreased(P＜0.05).Conclusion Ulinastatin exerted protective and therapeutic effects on NEC in neonatal rats.关键词
坏死性小肠结肠炎/乌司他丁/Shiou评分/自噬与凋亡/炎性因子Key words
Necrotizing enterocolitis/Ulinastatin/Shiou score/Autophagy and apoptosis/Inflammatory factors引用本文复制引用
赖盼建,徐洁,李小兵,包云光..乌司他丁对坏死性小肠结肠炎新生大鼠小肠结肠的保护作用研究[J].浙江医学,2017,39(20):1735-1737,1741,后插1,5.基金项目
浙江省科技计划实验动物项目(2014C37024) （2014C37024）
金华市科技计划项目(2015-3-010) （2015-3-010）
