浙江医学2017，Vol.39Issue(20)：1738-1741,4.DOI:10.12056/j.issn.1006-2785.2017.39.20.2017-377
线粒体Omi/HtrA2信号通路参与人脑挫裂伤后神经细胞凋亡的研究
Mitochondrial Omi /HtrA2 signaling pathway involved in neuronal apoptosis after cerebral contusion and laceration
摘要
Abstract
Objective To investigate the effect of Omi/HtrA2 mediated mitochondrial signaling pathway in neuronal apoptosis after acute traumatic brain injury.Methods Brain tissue samples were collected during craniotomy from 100 patients with cerebral contusion and laceration (trauma group),and normal brain tissue samples were collected from 100 patients with intracerebral hemorrhage(control group).Flow cytometry was applied to determine apoptotic neuronal cells,Western-blotting was performed to test expressions of Omi/HtrA2,X-linked inhibitor of apoptosis protein (XIAP),pro-caspase-3,pro-caspase-9 and cleaved poly ADP-ribose polymerase (PARP),and four peptide fluorescence substrate method was employed to detect activities of caspase 3 and caspase 9 proteins.Results Percentage of apoptotic nerve cells,expressions of Omi/HtrA2,pro-caspase 3,pro-caspase 9 and cleaved PARP as well as activities of caspase 3 and caspase 9 proteins were significantly higher and the expression of XIAP was significantly lower in brain tissues of trauma group than those in control group (all P＜0.01).In trauma group,expression of Omi/HtrA2 was positively correlated with percentage of apoptotic nerve cells,expressions of pro-caspase 3,pro-caspase 9 and cleaved PARP as well as activities of caspase 3 and caspase 9 proteins,and negatively correlation with the expression of XIAP.The expression of XIAP was negatively correlated with percentage of apoptotic nerve cells,expressions of Omi/HtrA2,pro-caspase 3,pro-caspase 9 and cleaved PARP as well as activities of caspase 3 and caspase 9 proteins.Conclusion Omi/HtrA2 mediated mitochondrial signaling pathway may be involved in neuronal apoptosis after acute traumatic brain injury.关键词
人类/创伤性脑损伤/神经细胞凋亡/Omi/HtrA2Key words
Human/Traumatic brain injury/Neuronal apoptosis/Omi/HtraA2引用本文复制引用
汤蓓,石键,周玲,沈红卫,吴慧平,胡佳元,郑绍俭..线粒体Omi/HtrA2信号通路参与人脑挫裂伤后神经细胞凋亡的研究[J].浙江医学,2017,39(20):1738-1741,4.基金项目
浙江省医药卫生科技计划平台项目(2014ZDA019) （2014ZDA019）
