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妊娠晚期3种自体血贮存方法对红细胞凝集状态的影响

丁杰锋 陈海江 桑列勇 吴云路 杨旦

浙江医学2017,Vol.39Issue(20):1771-1773,1795,后插2,5.
浙江医学2017,Vol.39Issue(20):1771-1773,1795,后插2,5.DOI:10.12056/j.issn.1006-2785.2017.39.20.2016-2109

妊娠晚期3种自体血贮存方法对红细胞凝集状态的影响

Comparison of three different methods for autologous blood storage in late pregnant women

丁杰锋 1陈海江 2桑列勇 3吴云路 1杨旦2

作者信息

  • 1. 312000 绍兴市妇幼保健院医学检验科
  • 2. 312000 绍兴市妇幼保健院输血科
  • 3. 绍兴市中心血站
  • 折叠

摘要

Abstract

Objective To compare three different methods for autologous blood storage in late pregnant women.Methods One hundred and six pregnant women with indications of autotransfusion from January 2011 to June 2014 were enrolled.The autologous blood samples were collected and stored using three different methods:standard anticoagulation,extended anticoagulation and autologous components.The aggregation degree of red cells was observed under microscope (10 × 50) after 24h,7,14 and 21d of storage.Two hundred red cells of each group were observed and the number of free red ceils was counted.Results The red blood cells in standard anticoagulation group began rouleau-like agglutination at d3 and the number of free red cells decreased dramatically;the red blood cells in extended anticoagulation began rouleau-like agglutination at d7 and the number of free red cells decreased dramatically at d3;while in autologous components group,nearly all red cells were free from 24h to 21d after storage.Conclusion The separation of the blood component is the best method for storage of autologous blood in late pregnancy.

关键词

妊娠晚期/自体输血/标准抗凝/扩大抗凝/自体成分血

Key words

Late pregnancy/Autotransfusion/Standard anticoagulation/Extended anticoagulation/Autologous blood components

引用本文复制引用

丁杰锋,陈海江,桑列勇,吴云路,杨旦..妊娠晚期3种自体血贮存方法对红细胞凝集状态的影响[J].浙江医学,2017,39(20):1771-1773,1795,后插2,5.

基金项目

浙江省卫生适宜技术成果转化计划项目(2016ZHB030) (2016ZHB030)

浙江省医药卫生科技计划项目(2012KYB216) (2012KYB216)

浙江医学

OACSTPCD

1006-2785

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