制冷学报2017，Vol.38Issue(6)：111-118,8.DOI:10.3969/j.issn.0253-4339.2017.06.111
人肝癌细胞Hep⁃G2的冷冻干燥保存初探
Preliminary Research on Freeze?drying Preservation of Human Hepatoma Hep?G2 Cells
摘要
Abstract
Freeze?drying is a long?term and effective method for cell preservation. The lyoprotectant can have a significant effect on cell freeze?drying. In this study, different volume concentrations of Me2 SO, glycerol, and different mass concentrations polyvinylpyrrolidone ( PVP) , compound lyoprotectant for lyophilizing human Hepatoma Hep?G2 cells were considered. The effect of intracellular trehalose on the lyophilized cells was investigated by determining the cell?recovery rate, survival rate, and 24 h attachment rate. The results show that, compared with the control group, the cells added in a 40％ PVP ( w/v) + 10％ ( v/v) glycerol + 15％ fetal bovine serum ( FBS) ( v/v) + 20％ trehalose ( w/v ) solution received better protection. The cell recovery rate, survival rate, and 24 h adherent rate are 29. 58％, 42. 18％, and 18. 71％, respectively. When the extracellular trehalose molar concentration is 800 mmol/L, the Hepatoma Hep?G2 cell?recovery rate, survival rate, and 24 h adherent rate are 27. 81％, 66. 65％, and 33. 68％, respectively. It is significantly better than the control group and has the best protection for cell freeze?drying.关键词
冷冻干燥/冻干保护剂/细胞/存活率Key words
freeze⁃drying/lyoprotectant/cell/survival rate分类
通用工业技术引用本文复制引用
宋萍,李维杰,周新丽,刘宝林..人肝癌细胞Hep⁃G2的冷冻干燥保存初探[J].制冷学报,2017,38(6):111-118,8.基金项目
国家自然科学基金(51376132)资助项目. (The project was supported by the National Natural Science Foundation of China (No. 51376132).) （51376132）
