作物学报2017，Vol.43Issue(12)：1746-1759,14.DOI:10.3724/SP.J.1006.2017.01746
不同磷水平下大麦分蘖期磷效率相关性状QTL定位分析
Mapping QTLs for Phosphorus Efficiency at Tillering Stage under Different Phosphorus Levels in Barley (Hordeum vulgare L.)
摘要
Abstract
Phosphorus (P) nutrition has close relationship with the quality and yield of barley and the genetic mechanism of P-efficient and variety improvement become hot research topics in recent years. In this study, we mapped quantitative trait loci (QTLs) for P-efficient traits using a recombinant inbred line (RIL) population derived fro ma cross between Baud in and CN4079. P-utilization efficiency (PUE), P-absorption efficiency (PAE), and dry weight (DW) of shoots and roots, as well as tiller number (TN) were evaluated in low-P (0.02 mmol L-1 KH2PO4) and normal-P (0.2 mmol L-1 KH2PO4) conditions. QTL sassociated with these traits were mapped onto a barley linkage map and their candidate genes were predicted. Phenotyping results showed con-tinuous variation and transgressive segregation in all traits tested. A total of 16 QTLs were detected on chromosomes 2H, 3H, and 5H under low-P and normal-P conditions, with explained phenotypic variance ranging from 14.1% to 28.5%. Three QTLs associ-ated with PUE were mapped on 3H and their positive alleles were all from Baud in. Among them, Qspue.sau-3H.1 and Qrpue.sau-3H were located in common region with PAE loci Qspae.sau-3H and Qrpae.sau-3H, whereas Qspue.sau-3H.2 was very closely to Qtn.sau-3H controlling TN. Three QTLs for PAE were mapped on 5H, among which Qspae.sau-5H.2 and Qrpae.sau-5H were from CN4079. The two PAE loci were located in adjacent region intervals of PUE loci Qspue.sau-5H and Qrpue.sau-5H and DW loci Qsdw.sau-5H and Qrdw.sau-5H. In the four chromosomal fragments harboring P-efficiency loci, ex-cept for Qspue.sau-3H.1 that contained candidate genes in phosphoric acid metabolism and phospholipid metabolism, the remaining loci all containedgenes in phosphate transporter and related genes in phosphoric acid metabolism and phospholipid metabolism.关键词
大麦/磷效率/分蘖期/重组自交系/QTL定位Key words
Hordeum vulgare L./Tillering stage/Phosphorus efficiency/RIL/QTL mapping引用本文复制引用
胡德益,蔡露,陈光登,张锡洲,刘春吉..不同磷水平下大麦分蘖期磷效率相关性状QTL定位分析[J].作物学报,2017,43(12):1746-1759,14.基金项目
本研究由国家自然科学基金项目(31401377), 四川省科技支撑计划项目(2014NZ0008)和四川省教育厅重点项目(14ZA0002)资助.This study was supported by the National Natural Science Foundation of China (31401377), the Key Technology R&D Program of Sichuan Province (2014NZ0008), and the Key Project of Education Department of Sichuan Province (14ZA0002). （31401377）
