作物学报2017，Vol.43Issue(12)：1760-1766,7.DOI:10.3724/SP.J.1006.2017.01760
糯玉米opaque2基因近等基因系的创制
Construction of Waxy Maize opaque2 Near-isogenic Lines
摘要
Abstract
In waxy maize, the opaque2 gene can increase the content of essential amino acids, such as lysine and tryptophan. Two high quality protein maize (QPM) inbred lines, CA339 and Lu 2548, were used as opaque2 gene donors. Twenty-five waxy maize with advantageous traits were used as receptors. Application of backcrossing and SSR marker-assisted selection in breeding can create waxy maize opaque2 near-isogenic lines. The results of molecular marker indicated that there were polymorphisms in dif-ferent receptors and between the two donors. Lysine content of five sets of successfully created opaque2 near-isogenic lines was tested, showing that the content of lysine in o2w-NILs was 59.0%, 52.7%, 48.5%, 46.3%, and 61.9% higher than that in their re-current parents, which was increased from 0.308%, 0.313%, 0.309%, 0.341%, 0.323% to 0.489%, 0.478%, 0.458%, 0.498%, and 0.522%, respectively. Using this method, opaque2 could be introgressed into multiple receptors with different genetic back-grounds to select near-isogenic lines with high lysine content and transparent phenotype, so as to improve nutritional and eco-nomic values of waxy maize.关键词
糯玉米/opaque2基因/waxy基因/近等基因系/赖氨酸Key words
Waxy maize/Opaque2 gene/Waxy gene/Near-isogenic lines/Lysine引用本文复制引用
张晓星,朱慧,张东民,宋丽雅,张德贵,翁建峰,郝转芳,李明顺..糯玉米opaque2基因近等基因系的创制[J].作物学报,2017,43(12):1760-1766,7.基金项目
本研究由国家自然科学基金项目(31401390)和国家现代农业产业技术体系建设专项(CAR02-01)资助.This study was supported by the National Natural Science Foundation of China (31401390) and the China Agriculture Research System (CAR02-01). （31401390）
