作物学报2017，Vol.43Issue(12)：1767-1773,7.DOI:10.3724/SP.J.1006.2017.01767
LAZY1通过油菜素内酯途径调控水稻叶夹角的发育
LAZY1 Regulates Development of Rice Leaf Angle through Brassinolide Pathway
摘要
Abstract
The angle of rice leaf, as an important agronomic trait, influences leaf area index and yield in rice. Leaf angle mutant identification is of significant importance in rice breeding based on ideal plant architecture. A mutant s524, derived from the progeny of EMS-treated indica rice Xida 1B, showed significantly larger leaf angle than the wild type. At tillering stage, the tiller angle and leaf angle of s524 increased and the whole plant crept, while the wild type in whole growth period maintained relatively compact phenotype. Longitudinal sections of the epidermis of pulvinus showed that the enlarged leaf angle of the mutant was mainly due to the much-elongated adaxial cells. The main agronomic traits of s524 did not change significantly compared with those of the wild-type. Genetic analysis suggested that the mutational traits were controlled by a recessive nuclear gene, which was finely mapped between SSR markers RM4746 and RM26742 on chromosome 11 with a physical distance of 324 kb. LAZY1 in the restricted region regulates rice tiller angle development. Sequencing revealed that a base substitution from T to C occurred on the third exon of LAZY1, leading to the amino acid change from valine to alanine at 143th protein sequence. Hormone treat-ment test indicated that BR sensitivity was reduced in s524. Quantitative analysis demonstrated that the expression of BR signal-ing related gene BU1 was obviously up-regulated. The previous study showed that over-expression of BU1 could enlarge rice leaf angle. Our research suggests that LAZY1/S524 may regulate the development of leaf angle through BR conduction pathway.关键词
水稻(OryzasativaL.)/叶夹角/LAZY1/S524/油菜素内酯(BR)信号传导/BU1Key words
Rice (Oryza sativa L.)/Tiller angle/LAZY1/S524/Signal transduction of brassinolide (BR)/BU1引用本文复制引用
张晓琼,王晓雯,田维江,张孝波,孙莹,李杨羊,谢佳,何光华,桑贤春..LAZY1通过油菜素内酯途径调控水稻叶夹角的发育[J].作物学报,2017,43(12):1767-1773,7.基金项目
本研究由中央高校基本科研业务费(XDJK2015C117)和重庆市基础科学与前沿技术研究(一般)项目(cstc2015jcyjA1517)资助.This study was supported by the Fundamental Research Funds for the Central Universities (XDJK2015C117) and Chongqing Research Program of Basic Research and Frontier Technology (cstc2015jcyjA1517). （XDJK2015C117）
