作物学报2017，Vol.43Issue(12)：1791-1801,11.DOI:10.3724/SP.J.1006.2017.01791
海岛棉枯萎病抗性与类黄酮代谢途径基因表达量的相关性
Correlation between Fusarium wilt Resistance and Expression Levels of Genes Involved in Flavonoid Metabolism Pathway in Gossypium barbadense L.
摘要
Abstract
Fusarium wilt is one of the important factors threatening the production of island cotton (Gossypium barbadense L.). Understand the molecular mechanism of Fusarium wilt resistance will facilitate cotton breeding against Fusarium wilt and there-fore alleviate the problem in cotton production. On the basis of RNA-seq results in previous studies, differentially expressed genes (DEG) were further analyzed in this study. After inoculating the pathogenic fungus for 0–40 hours, the DEGs expression levels of seven varieties in different wilt resistance were studied using qRT-PCR method. The correlation between gene expression level and disease index was analyzed. The flavonoid biosynthetic pathway genes were found to be related to Fusarium wilt resistance. The qRT-PCR result indicated the significantly higher expression levels of flavonoid metabolic pathway genes in resistant geno-types than those susceptible genotypes. The expression levels of TT7, CHI, and DFR, the key genes in flavonoid metabolic path-ways, were significantly higher in resistant genotypes than in susceptible genotypes (P < 0.05 or P < 0.01) at several time points after inoculation. Particularly, the expression levels of CHI and DFR were negatively correlated with disease index. Therefore, we conclude that the flavonoid metabolic pathway related genes might be involved in the resistance to Fusarium wilt in cotton, among which CHI, TT7, and DFR are the key genes.关键词
海岛棉/枯萎病/类黄酮/表达分析/转录组测序Key words
Gossypium barbadense/Fusarium wilt/Flavonoids/Expression analysis/RNA-seq引用本文复制引用
黄启秀,曲延英,姚正培,李梦雨,陈全家..海岛棉枯萎病抗性与类黄酮代谢途径基因表达量的相关性[J].作物学报,2017,43(12):1791-1801,11.基金项目
本研究由国家自然科学基金项目(31560409)资助.This study was supported by the National Natural Science Foundation of China (31560409). （31560409）
