北京林业大学学报2017，Vol.39Issue(12)：71-78,8.DOI:10.13332/j.1000-1522.20170194
不同世代樟子松育种资源遗传评价
Genetic evaluation of breeding resources of Pinus sylvestris var. mongolica from different improved generations.
摘要
Abstract
Nowadays, the genetic improvement of Pinus sylvestris var. mongolica is in a critical period of upgrading and updating. But the frequent allocation and exchange of breeding resources in different areas lead to unclear information of breeding resources and confusion of genetic relationship, which increases the risk of inbreeding depression during genetic improvement of Pinus sylvestris var. mongolica. Therefore, the evaluation of breeding resources is an important basis for the utilization of superior individuals of Pinus sylvestris var. mongolica. In this paper, 224 clones of Pinus sylvestris var. mongolica from the 1st,1. 5th and 2nd seed orchards were studied. Genetic diversity, phylogenetic relationship and genetic diversity were analyzed based on SSR, and the population of BaogenYuan and Yulin was relatively low according to breeding resource analysis;the genetic diversity of four populations of LiangZhongJiDi, TouDaoQiao, YuLin1. 5 and TaiLai was higher. According to improved generation analysis, different generations of Pinus sylvestris var. mongolica breeding resources maintained a high level of genetic diversity; the genetic diversity of the high generation seed orchard decreased, and the expected heterozygosity ( He ) of the 2 nd and 1. 5 th generation seed orchards decreased by 4. 13% and 1. 31%respectively compared with the 1 st generation seed orchard; the 2 nd generation seed orchard group had more higher inbreeding coefficient (the F-value was 0. 173) and homozygote, which significantly deviated from Hardy-Weinberg equilibrium;Pinus sylvestris var. mongolica breeding resources in different habitats and the same source had no obvious variation, the genetic variation mainly existed among individuals (96%);with the generation seed orchard increased, phylogenetic relationship clustering phenomenon among clones gradually became obvious, two sets of breeding resource clones were identified completely genetic consistent in the 2nd generation seed orchard. The results of this research improve the breeding resource evaluation system of Pinus sylvestris var. mongolica, determine the genetic relationship of different generation breeding resources, and provide the material and information basis for the advanced genetic improvement work of Pinus sylvestris var. mongolica.关键词
樟子松/育种资源/遗传评价/亲缘关系Key words
Pinus sylvestris var. mongolica/breeding resource/genetic evaluation/phylogenetic relationship分类
农业科技引用本文复制引用
苗禹博,朱晓梅,李志娟,贾凤岭,李伟..不同世代樟子松育种资源遗传评价[J].北京林业大学学报,2017,39(12):71-78,8.基金项目
国家重点研发计划项目(2017YFD0600505). （2017YFD0600505）
