草业学报2017，Vol.26Issue(12)：98-107,10.DOI:10.11686/cyxb2017054
低温对青海扁茎早熟禾活性氧代谢的影响和相关基因表达分析
Effect of freezing stress on ROS metabolism and cold-induced genes in Poa pratensis var.anceps cv.Qinghai
摘要
Abstract
To explore the mechanism of cold tolerance of Poa pratensis var.anceps cv.Qinghai (BJ) plants were exposed to cold acclimation (10/5 ℃) for 3 d followed by freezing stress (-10 ℃) for 24 h,with P.pratensis var.'Midnight Ⅱ' (WY-2) as control,and ROS accumulation, antioxidant enzyme activity and stress related gene expression were monitored.Rate of the production of O2-·,levels of H2O2 and MDA,activity of SOD,CAT,APX,GR and the relative expression of the antioxidant enzyme genes were measured,as well as several cold induced genes.The results demonstrated that freezing stress increased the O2-· production rate,and H2O2 and MDA levels in both BJ and WY-2.ROS accumulation and MDA content of BJ were lower than those in WY-2.SOD,APX and GR activity was depressed by freezing stress in BJ,while CAT activity initially increased and then decreased.Only APX in BJ showed higher activity than that in WY-2.At the same time,the expression levels of Cu/ZnSOD,CAT,APX and GR related genes were increased in the two varieties.The relative expression of the enzyme related genes in BJ was higher than WY-2.The cold induced gene DREB2 was down-regulated in BJ,while the ERF,DREB1,CSP,and HSP genes were up-regulated.Among these genes,ERF,CSP and HSP showed higher expression levels in BJ than in WY-2.In conclusion,the accumulation of ROS in BJ was lower than that in WY-2 under cold and freezing stress.Meanwhile antioxidant enzyme related genes and cold related transcription factors showed higher relative expression levels in BJ than in WY-2 under cold and freezing stress.Those differences may contribute to the higher cold tolerance of BJ.关键词
青海扁茎早熟禾/草地早熟禾/活性氧/抗氧化酶/基因表达Key words
Poa pratensis var.anceps cv.Qinghai/kentucky bluegrass/ROS/antioxidant enzyme/gene expression引用本文复制引用
史毅,牛奎举,余倩倩,刘文辉,马晖玲..低温对青海扁茎早熟禾活性氧代谢的影响和相关基因表达分析[J].草业学报,2017,26(12):98-107,10.基金项目
国家自然科学基金(31160482)资助. （31160482）
