草业学报2017，Vol.26Issue(12)：117-127,11.DOI:10.11686/cyxb2017208
过表达FaSAMDC基因提高黑麦草属植物的抗旱性和耐热性
Enhancing drought and heat tolerance in Lolium spp.through overexpression of the FaSAMDC gene
摘要
Abstract
To obtain new ryegrass germplasm with enhanced drought and heat tolerance a genetic transformation experiment using plants from the genus Lolium was conducted.By using mature seeds of ryegrass as explants and adoption of the overexpression vector,tissue culture and regeneration system of ryegrass,and genetic transformation mediated by Agrobacterium tumefaciens,the S-adenosylmethionine decarboxylase gene cloned from Festuca arundinacea (FaSAMDC) was introduced into the genome of 'Tetragold' annual ryegrass and 'Four Seasons' perennial ryegrass.Eighty-nine transgenic plants (45 Tetragold and 44 Four Seasons) were regenerated from independent resistant calli,which were confirmed by antibiotic resistance screening,PCR assay and southern hybridization analysis;the transgenic frequency was 2.93％ and 2.28％,respectively.Drought and heat resistant tests under high temperature (30 ℃) and moderate drought stress showed that in Tetragold,the root to shoot ratio was 7.1％ higher,leaf relative water content (RWC) 13.1％ higher and the RWC descent scope 5.5％ lower than the controls;for transgenic Four Seasons plants the root to shoot ratio was 6.5％ higher,RWC 12.5％ higher and the RWC descent scope 6.5％ lower than the control;differences between transgenic plants and controls were all statistically significant (P＜0.05),indicating that drought and heat resistance in transgenic plants was significantly improved.Observations of morphological and growth characteristics showed that,compared with non-transgenic plants,leaf length,plant height and main stalk pitch number of transgenic plants were lower but leaf width and tiller number per plant were higher.Leaves of transgenic plants were darker in colour and plants tended to become compact with tufted leaves suggesting that the inserted FaSAMDC gene was involved in the regulation of physiological function,such as gene expression and cell division.The transgenic material developed in the study could be used as foundation for the cultivation of new ryegrass varieties with enhanced drought and heat resistance.关键词
黑麦草/根癌农杆菌/遗传转化/FaSAMDC基因/抗旱耐热性Key words
ryegrass/Agrobacterium tumefaciens/genetic transformation/FaSAMDC gene/drought and heat tolerance引用本文复制引用
曾庆飞,韦鑫,蔡一鸣,舒健虹,吴佳海,王小利..过表达FaSAMDC基因提高黑麦草属植物的抗旱性和耐热性[J].草业学报,2017,26(12):117-127,11.基金项目
贵州省农业科技攻关项目(黔科合NY[2013]3060号),贵州省农业科学院博士科研启动基金(2013-06),黔农科院自主创新科研专项[(2014)010号],贵州省高层次创新型人才培养项目(黔科合人才[2016]4024)和贵州省科技创新人才团队建设项目(黔科合平台人才[2016]5617)资助. （黔科合NY[2013]3060号）
