草业学报2017，Vol.26Issue(12)：128-137,10.DOI:10.11686/cyxb2017130
草地早熟禾干旱胁迫转录组差异性分析
Differential gene analysis of Poa pratensis in response to drought stress
摘要
Abstract
Drought is one of the main factors affecting the productivity of Poa pratensis.This study was undertaken to reveal the change of gene expression profile of P.pratensis in the absence of a reference genome.The high-throughput Illumina Hiseq sequencing platform was used to investigate the transcriptome of a control group (CK) and a drought treated group (D) of P.pratensis.The sequencing data were subsequently analyzed to help reveal the molecular mechanisms of drought response in P.pratensis.The results showed that 24465 differentially expressed genes were detected under drought treatment.After screening 4143 up-regulated genes and 4415 down regulated genes were obtained,accounting for 34.98％ of the total number of differentially expressed genes.The genes related to protein kinase,protein phosphatase,carbon metabolism and ABA can be used as the main research object to study the drought response mechanism of P.pratensis,qRT-PCR analysis showed that the expression of 8 randomly differentially expressed genes was consistent with the high-throughput sequencing results.In addition,a series of genes for indole-3-glycerophosphate synthase,protein phosphatase,carbohydrate kinase,calcium binding protein and chlorophyll a/b binding protein were selected as candidate genes for drought stress related to P.pratensis.This laid the foundation for potentially revealing the molecular mechanism of drought response in P.pratensis.关键词
草地早熟禾/干旱胁迫/高通量测序/转录组Key words
Poa pratensis/drought stress/high-throughput sequencing/transcription group引用本文复制引用
冷暖,刘晓巍,张娜,许立新..草地早熟禾干旱胁迫转录组差异性分析[J].草业学报,2017,26(12):128-137,10.基金项目
中国林学会——青年人才托举工程项目资助. （）
