重庆医学2017，Vol.46Issue(36)：5047-5048,5051,3.DOI:10.3969/j.issn.1671-8348.2017.36.003
螺旋藻激酶对急性血瘀模型大鼠的影响
The effects of Spirulina kinase in rats with acute blood stasis
摘要
Abstract
Objective In this study ,the effect of Spirulina kinase(SPK)on hemorheology ,platelet aggregation and cAMP in acute blood stasis rats model were investigated .Methods Forty SD rats were randomly divided into normal control group ,model group ,aspirin group and the high and low dose of SPK groups .All treatments were performed by gavage once a day for 7 days .Sub-cutaneous injection of adrenalin combined with ice water bath were used to establish the acute blood stasis rat model .The whole blood viscosity ,plasma viscosity ,red blood cell hematocrit (Hct) ,erythrocyte aggregation index (EAI) ,red blood cell deformation index(DI)anderythrocyterigidityindex(ERI)weredetectedbyautomaticbloodflow speedanalyzer .Meanwhile,thelevelofcAMP was detected by ELISA method .Rat platelet aggregation induced by ADP and the maximum aggregation rate was measured by tur-bidimetry .Results Results showed that SPK could significantly decrease the whole blood viscosity ,plasma viscosity ,Hct ,EAI and platelet aggregation rate ,increase the level of cAMP compared with model group(P<0 .05) ,but had no effect on DI and ERI .Con-clusion SPK can markedly improve the abnormal changes of hemorheology in acute blood stasis rats and inhibit the platelet aggregation .关键词
螺旋藻激酶/血瘀/血液流变学/血小板聚集/环磷酸腺苷Key words
Spirulina kinase/blood stasis/hemorheology/platelet aggregation/cAMP分类
医药卫生引用本文复制引用
黄媛恒,覃斐章,李映新,原春兰,蒋丽梅,王琳,庞辉..螺旋藻激酶对急性血瘀模型大鼠的影响[J].重庆医学,2017,46(36):5047-5048,5051,3.基金项目
广西自然科学基金资助项目(2014GXNSFBA118156,2014GXNSFBA118188 ,2013GXNSFAA019176) （2014GXNSFBA118156,2014GXNSFBA118188 ,2013GXNSFAA019176）
2013年广西壮族自治区区级大学生创新创业训练计划项目(桂教高教[2013]14号). （桂教高教[2013]14号）
