重庆医学2017，Vol.46Issue(36)：5049-5051,3.DOI:10.3969/j.issn.1671-8348.2017.36.004
Wnt3a通过整合素连接激酶调节血管平滑肌细胞迁移和黏附
Wnt3a regulates migration and adhesion of vascular smooth muscular cells via integrin-linked kinase
摘要
Abstract
Objective To investigate the effects and related mechanism of recombinant Wnt 3a protein on vascular smooth muscle cells(VSMCs) migration and adhesion .Methods Primary rat VSMCs were cultured .The experiment was divided into the Wnt3a group and control group .The VSMC migration ability was detected by Transwell experiment .And the ability of VSMC ad-hesion to extracelluar matrix was detected by the celluar matrix adhesion assay .The protein expression levels of β-catenin ,phos-phor-β-catenin (Ser675) ,GSK-3β,phosphor-GSK-3β(Ser9) and integrin-linked kinase(ILK) in VSMCs were detected by Western blot and analyzed .Results The number of VSMCs migration in the Wnt3a group was significantly higher than that in the control group (P<0 .05) .Compared with the control group ,the number of VSMCs adhesion to collagen Ⅰ and the optical density value in the Wnt3a group were significantly increased (P<0 .05) ,and the protein expression levels of β-catenin ,Ser675 ,GSK-3β,Ser9 and ILK in the Wnt3a group were significantly up-regulated (P<0 .05) .Conclusion Wnt3a can regulate VSMCs migration and adhesion by ILK .关键词
Wnt蛋白质类/肌,平滑,血管/细胞运动/细胞黏附/蛋白质丝氨酸苏氨酸激酶Key words
Wnt proteins/muscle/smooth/vascular/cell movement/cell adhesion/protein-serine-threonine kinases分类
医药卫生引用本文复制引用
吴校林,朱锐,李彬,刘文卫,周青,赵玉勤..Wnt3a通过整合素连接激酶调节血管平滑肌细胞迁移和黏附[J].重庆医学,2017,46(36):5049-5051,3.基金项目
湖北省自然科学基金青年项目(2016CFB344). （2016CFB344）
