南方医科大学学报2017，Vol.37Issue(12)：1563-1569,7.DOI:10.3969/j.issn.1673-4254.2017.12.01
ABCA1敲低对小鼠巨噬细胞中Pam3CSK4引起的炎症反应的双向调节作用
Bidirectional regulation of Pam3CSK4-induced inflammatory response by ATP-binding cassette transporter A1 knockdown in mouse mononuclear macrophages in vitro
摘要
Abstract
Objective To investigate the regulatory effect of ATP-binding cassette transporter A1 (ABCA1) knockdown on inflammatory response induced by Pam3CSK4 in mouse mononuclear macrophage RAW264.7 cell line. Methods A mouse mononuclear macrophage RAW264.7 cell line with stable ABCA1 knockdown was constructed and stimulated with Toll-like receptor 2 (TLR2) ligand Pam3CSK4, and the changes in the transcriptional levels of the proinflammatory and anti-inflammatory cytokines were analyzed in this cell model. Results In RAW264.7 cells, ABCA1 knockdown significantly up-regulated Pam3CSK4 stimulation-induced expressions of IL-1β, TNF-α and IL-6 and also enhanced the expression of transcription factor cAMP-dependent transcription factor 3 (ATF3) without obviously affecting the expressions of the transcription factors ATF1, ATF2, ATF4 or ATF5. Conclusion ABCA1 knockdown in macrophages may have both proinflammatory and anti-inflammatory effects.ABCA1 knockdown up-regulates the transcription of ATF3 possibly through a mechanism that is different from that for the other members of the ATF protein family.关键词
ABCA1/TLR2/ATF3/Pam3CSK4Key words
ATP-binding cassette transporter A1/Toll-like receptor 2/activating transcription factor 3/Pam3CSK4引用本文复制引用
李玥,蓝茜,武丽涛,杜小娟,Ezra Kombo Osoro,李冬民,吕社民..ABCA1敲低对小鼠巨噬细胞中Pam3CSK4引起的炎症反应的双向调节作用[J].南方医科大学学报,2017,37(12):1563-1569,7.基金项目
国家自然科学基金(81370952) （81370952）
陕西省科技计划项目(2013K21-22-03)Supported by National Natural Science Foundation of China(81370952). （2013K21-22-03）
