南方医科大学学报2017，Vol.37Issue(12)：1570-1576,7.DOI:10.3969/j.issn.1673-4254.2017.12.02
白细胞介素-22促进类风湿关节炎成纤维化膜细胞的增殖
Interleukin-22 promotes proliferation of fibroblast-like synoviocytes from patients with rheumatoid arthritis by inducing STAT3 phosphorylation
摘要
Abstract
Objective To clarify the mechanism by which interleukin-22 (IL-22) promotes the proliferation of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). Methods FLS were isolated from the synovial tissues of patients with RA and identified by immunohistochemistry for vimentin/CD68.The cells were subcultured and incubated with different concentrations of IL-22 for 24,48,or 72 h,and their proliferation was examined using MTT assay.After treatment of the cells with IL-22 and AG490,alone or in combination,the expressions of the total and phosphorylated proteins of STAT3, ERK1/2 and P38 were detected with Western blotting. Results IL-22 significantly increased the proliferation of FLS in a dose-dependent manner(P<0.05).The total protein of STAT3 in the cells showed no significant changes with extended time of IL-22 treatment(P=0.68),but the expression of phosphorylated STAT3 protein increased significantly(P<0.001).The total and phosphorylated proteins of ERK1/2 and P38 underwent no significant changes after IL-22 treatment (P>0.05). A combined treatment with 50 ng/mL IL-22 and 100 μmol/L AG490 resulted in a significant decrease in the proliferation of FLS as compared with IL-22 treatment alone(P<0.01).Conclusion IL-22 can dose-dependently promote the proliferation of FLS from patients with RA by inducing phosphorylation of STAT3 protein but not through ERK1/2 or P38 signal pathway.关键词
类风湿关节炎/成纤维滑膜细胞/白细胞介素22/细胞信号通路Key words
rheumatoid arthritis/fibroblast-like synoviocytes/interleukin-22/signaling pathway引用本文复制引用
朱俊卿,王然,陈世贤,赵迪,李娟..白细胞介素-22促进类风湿关节炎成纤维化膜细胞的增殖[J].南方医科大学学报,2017,37(12):1570-1576,7.基金项目
国家自然科学基金/海外及港澳学者合作研究基金(81628017, 81573730) （81628017, 81573730）
广东省自然科学基金(2017A030313868) （2017A030313868）
南方医院院长基金(2016C024) （2016C024）
南方医科大学科研启动计划(QD2017N022)Supported by National Natural Science Foundation of China(81628017, 81573730). （QD2017N022）
