南方医科大学学报2017，Vol.37Issue(12)：1660-1666,7.DOI:10.3969/j.issn.1673-4254.2017.12.17
新西兰兔再生障碍性贫血模型的建立
Establishment of New Zealand rabbit models of aplastic anemia
摘要
Abstract
Objective To screen for the optimal dose of benzene and cyclophosphamide using an orthogonal design for establishment of New Zealand rabbit models of aplastic anemia.Methods Following an orthogonal experimental design,the effects of 3 levels of 4 factors, namely the dose of benzene (A), the dose of cyclophosphamide (B), the number of benzene injections (C), and the number of cyclophosphamide injections (D) were tested in the establishment of New Zealand rabbit models of aplastic anemia using a L9(34)orthogonal table,and the optimal protocol for the model establishment was selected from the 9 experimental groups.Each rabbit received subcutaneous injection of benzene on the back every other day,followed by daily cyclophosphamide injection via the ear vein for prescribed times.The blood routine was examined every 6 days,and before modeling and at 36 days after modeling, a small sample of the femoral bone was collected for bone marrow histopathological examination. Results Comparison of the white blood cell, erythrocyte and platelet counts among the 9 groups showed successful modeling in Groups 4-9, and daily mean reduction rates of the cell counts in Groups 7, 8, and 9 differed significantly from those in the other groups(P<0.05).In Group 7,bone marrow sections showed low myelodysplasia, reduced hematopoietic tissue,reduced or even absence of megakaryocytes,and increased fat cells.Further observation found that the rabbits in Group 7 had sustained bone marrow suppression,consistent with the clinical characteristics of the disease. Conclusion Stable models of aplastic anemia can be established efficiently in New Zealand rabbits by a combination of 8 subcutaneous injections of benzene at 1.5 mL/kg and 4 intravenous injections of cyclophosphamide at 10 mg/kg.关键词
再生障碍性贫血/苯/环磷酰胺/正交试验/动物模型Key words
aplastic anemia/benzene/cyclophosphamide/orthogonal test/animal models引用本文复制引用
罗东,罗月苹,刘宝茹,梁丹丹,蒋璟玮,汪威,陈俊林,王嫣,陈文直..新西兰兔再生障碍性贫血模型的建立[J].南方医科大学学报,2017,37(12):1660-1666,7.基金项目
重庆市前沿与应用基础研究计划一般项目(cstc2016jcyjA0599) （cstc2016jcyjA0599）
