南方医科大学学报2017，Vol.37Issue(12)：1688-1694,7.DOI:10.3969/j.issn.1673-4254.2017.12.22
不同受压时间窗及干预方式对压疮大鼠模型皮肤损伤及缺血再灌注的影响
Effect of different time windows and interventions on skin pressure ulcers and ischemia-reperfusion injury in rats
摘要
Abstract
Objective To observe the effect of different time windows and interventions on skin pressure ulcers and ischemia-reperfusion(I/R)injury in rats.Methods Sixty-eight SD rats were randomly divided into blank control group(n=4)and model group(n=64).The rats in the model group were randomly divided into group A(n=32)without intervention and group B(n=32) with post-conditioning. The degree of skin compression, neutrophil infiltration and serum levels of free radicals were observed in the rats after compression for 2,4,6,and 8 h(8 rats at each time point).Results A significant difference was found in the severity of skin damage among the control group,group A,and group B(P=0.001),and the injury was milder in group B than in group A.Severe skin lesions occurred in 2 rats after skin compression for 6 h,as compared with 6 after compression for 8 h(P=0.043),but in none of the rats after compression for 2 or 4.Seventeen rats in group B and 15 in group A showed grade 1 neutrophil infiltration in the skin lesions,and 8 rats in group B and 10 in group A showed grade II neutrophil infiltration(P=0.002).Neutrophil infiltration was the mildest in rats with a 2-h compression,and exacerbated progressively and significantly as the compression time extended(P=0.027).With the prolongation of the intervention time,the rats in both groups A and B showed decreased SOD and increased MDA and NO levels,and overall the I/R injury was milder in 2-and 4-h compression groups than in 6-and 8-h compression groups.The level of serum SOD was significantly higher and MDA and NO levels were significantly higher in group B than in group A(P<0.05).Conclusion Ischemic post-conditioning can relieve I/R injury in acute pressure ulcer in rats. The effective time window for intervention is within 6 h of ischemia, and the effect of ischemic post-conditioning is optimal within 2 h.Ischemic post-conditioning can alleviate free radical injury and inflammation caused by I/R injury.关键词
压疮/缺血再灌注/缺血后处理/时间窗/动物实验Key words
pressure ulcer/ischemia reperfusion/post-ischemic treatment/time window/animal experiment引用本文复制引用
王华军,戴世学,卢铨,叶林昌,李华,宋希,洪涛,沙卫红..不同受压时间窗及干预方式对压疮大鼠模型皮肤损伤及缺血再灌注的影响[J].南方医科大学学报,2017,37(12):1688-1694,7.基金项目
广东省医学科学基金(B2014009) （B2014009）
