分析化学2018，Vol.46Issue(1)：20-26,7.DOI:10.11895/j.issn.0253-3820.170114
一种基于香豆素衍生物的铁离子水溶性荧光探针的合成及其应用
Synthesis and Biological Application of A Water-Soluble Fluorescent Probe Based on Coumarin Derivatives for Detection of Ferric Ion
摘要
Abstract
A water-soluble fluorescent probe (7-diethyl amino-3-formaldehyde coumarin) for Fe3+ detection was designed and synthesiZed, and its structure was confirmed by 1 HNMR, 13 CNMR and MS spectra.This probe showed high emission intensity at 471 nm.With the continuous addition of Fe3+, the emission intensities at 471 nm decreased gradually, and showed an excellent linearity with Fe3+in the range of 0.02-60 μmol/L, and the regression equation was I=322.56-4.86CFe3+.This probe was able to detect Fe3+ qualitatively and quantitatively with the detection limit as low as 22 nmol/L.Besides, this probe showed high sensitivity to Fe3+over other metal ions.The detection process was reversible, which could be recycled for the Fe3+detection.In terms of good optical properties and the strong fluorescence in physiological pH, the probe was successfully applied in imaging Fe3+ of living Ramos cells.关键词
荧光探针/铁离子/水溶性/细胞成像Key words
Fluorescent probe/Ferric ion/Water solubility/Cell imaging引用本文复制引用
陈兆辉,李媛媛,韩娟,王赟,任彦鹏,陈桐,唐旭,倪良..一种基于香豆素衍生物的铁离子水溶性荧光探针的合成及其应用[J].分析化学,2018,46(1):20-26,7.基金项目
本文系国家自然科学基金项目(No.21576124)、江苏出入境检验检疫局科技计划项目(No.2016KJ51)和镇江市重点研发计划——社会发展项目(Nos.SH2015014,SH2016019)资助 This work was supported by the National Natural Science Foundation of China (No.21576124). （No.21576124）
