分析化学2018，Vol.46Issue(1)：39-47,9.DOI:10.11895/j.issn.0253-3820.171163
基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像
A F?rster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells
摘要
Abstract
Hydrogen sulfide ( H2S ) has been confirmed as a significant endogenous gaseous signaling molecule involved in various physiological processes.To monitor H2S in living cells, a F?rster resonance energy transfer ( FRET) ratiometric probe based on quantum dot-cresyl violet was developed.In this work, quantum dot nanospheres ( QDS) were firstly synthesiZed via a facile ultrasonication emulsion strategy, and the mixture chloroform solution containing hydrophobic quantum dots and COOH-functionaliZed amphiphilic polymer were successfully transferred into the oil-in-water micelle.The negatively charged quantum dot nanospheres with quantum dots embedded in the polymer matrixes were successfully fabricated after the evaporation of chloroform.And then, these quantum dot nanospheres were condensed with positively charged cresyl violet-aZide ( CV-N3 ) via electrostatic interaction to obtain the QDS-N3 complexes.The as-prepared QDS-N3 complexes were monodispersed nanospheres with an average diameter of about 120 nm.These complexes were taken up by the cell through endocytosis, and they were still stable even in wide pH range.In addition, the QDS-N3 complexes exhibited no cellular toxicity which was verified by MTT assay.In this ratiometric probe, CV-N3 as a FRET acceptor was conjugated to quantum dot nanospheres.The quantum dots emitted at 591 nm and served as the FRET donor;once the aryl aZide on the CV-N3 was reduced to aniline by H2S, the probe emitted at 620 nm.The ratiometric probe allowed the elimination of interference of excitation intensity, intracellular environment and other factors.Furthermore, this method also offered a general protocol for preparing nanosensors for monitoring various small molecular in living cells.关键词
量子点/甲酚紫/比率荧光/细胞成像/硫化氢Key words
Quantum dot/Cresyl violet/Ratiometric fluorescence/Cell imaging/Hydrogen sulfide引用本文复制引用
白敏,曹晓文,陈锋,赵越,赵永席..基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像[J].分析化学,2018,46(1):39-47,9.基金项目
本文系国家自然科学基金(Nos.21475102,31671013)和西安交通大学"青年拔尖人才计划"资助 This work was supported by the National Natural Science Foundation of China (Nos.21475102, 31671013)and the"Young Talent Support Plan" of Xi'an Jiaotong University, China. （Nos.21475102,31671013）
