分析化学2018，Vol.46Issue(1)：61-66,6.DOI:10.11895/j.issn.0253-3820.171014
金属/基质增强飞行时间-二次离子质谱用于单细胞脂质分析
Metal/Matrix Enhanced Time-of-Flight Secondary Ion Mass Spectrometry for Single Cell Lipids Analysis
摘要
Abstract
The chemical components analysis of single cell is important for understanding of physiological processes such as cell growth, signal transduction and apoptosis.Time-of-flight secondary ion mass spectrometry ( ToF-SIMS) is a sensitive surface analysis technique with high spatial resolution and can be used for single cell and micro-area analysis.However, relatively low ioniZation yield of biomolecules limited its wide application in single cell analysis.Herein, we used metal substrate and matrix material to enhance the ioniZation yield of lipids.The signal intensity of the phosphatidylcholine PC (40:0) casted on the matrix/gold coated silicon substrate was 65 times higher than that on the silicon wafer.Signal enhancement of phosphatidylcholine PC (34:1) on the single cell surface cultured on matrix/gold coated silicon substrate was observed as well.Due to the influence of irregular topography and complex chemical environment of cell, the increase of lipids signal was smaller.Delayed extraction mode of ToF-SIMS overcame the effects of cell topography, leading to further enhancement of the signal intensity of lipids.Meanwhile, simultaneous high spatial resolution of chemical imaging and high mass resolution of the mass spectra of single cells were obtained.Our strategies provided new insights into the study of cell metabolism and cell-environment interactions.关键词
单细胞分析/脂质/飞行时间-二次离子质谱/金属/基质增强/质谱成像Key words
Single cell analysis/Lipids/Time-of-flight secondary ion mass spectrometry/Noble metal/matrix enhancement/Mass spectrometry imaging引用本文复制引用
李好问,华鑫,龙亿涛..金属/基质增强飞行时间-二次离子质谱用于单细胞脂质分析[J].分析化学,2018,46(1):61-66,6.基金项目
本文系上海市青年科技英才扬帆计划(No.17YF1403000 )、上海市自然科学基金(No.17ZR1407700 )和中国博士后科学基金(No.2017M611470)资助 （No.17YF1403000 ）
