分析化学2018，Vol.46Issue(1)：113-120,8.DOI:10.11895/j.issn.0253-3820.171046
开放式微流控芯片上的肿瘤组织微阵列构建
Construction of Tumor Tissue Array on An Open-Access Microfluidic Chip
摘要
Abstract
An open-access microfluidic chip which enabled automatic cell distribution and complex multi-step operations was developed.The microfluidic chip featured a key structure in which a nanoporous membrane was sandwiched by a cell culture chamber array layer and a corresponding media reservoir array layer.The microfluidic approach took advantage of the characteristics of the nanoporous membrane.On one side, this membrane permitted the flow of air but not liquid, thus acting as a flow-stop valve to enable automatic cell distribution.On the other side, it allowed diffusion-based media exchange and thus, mimicked the endothelial layer.In synergy with a liquid transferring platform, the open-access microfluidic system enabled complex multi-step operations involving medium exchange, drug treatment, and cell viability testing.By using this microfluidic protocol, a 10 × 10 tissue arrays was constructed in 90 s, followed by schedule-dependent drug testing.Morphological and immunohistochemical assays results indicated that the resultant tumor tissue was faithful to that in vivo.Drug testing assays showed that the microfluidic tissue array promised multi-step cell assays under biomimetic microenvironment, thus providing an advantageous tool for cell research.关键词
开放式微流控芯片/纳米孔薄膜/三维细胞培养/组织微阵列/抗肿瘤药物测试Key words
Open-access microfluidic chip/Nanoporous membrane/Microenvironment/Tissue microarray/Anti-cancer drug testing引用本文复制引用
林冬果,林锦琼,李佩文,杨娜,徐邦牢,刘大渔..开放式微流控芯片上的肿瘤组织微阵列构建[J].分析化学,2018,46(1):113-120,8.基金项目
本文系国家自然科学基金(Nos.81371649, 81171418, 81602434)和广州市科创委协同创新项目(Nos.20141A010007, [2016]171)资助. This work was supported by the National Natural Science Foundation of China (Nos.81371649, 81171418, 81602434)and the GuangZhou Science Technology and Innovation Comission (Nos.20141A010007, [2016]171). （Nos.81371649, 81171418, 81602434）
