高等学校化学学报2018，Vol.39Issue(1)：48-53,6.DOI:10.7503/cjcu20170312
重组人促红细胞生成素-αDNA适配体的活性高级结构特征
Active Conformation of DNA Aptamer Against Recombinant Human Erythropoietin-α
摘要
Abstract
A series of spectroscopic techniques such as surface plasmon resonance, circular dichroism and fluorescence was employed to illustrate the relationship between the binding activity and conformation of the shortest aptamer In27 against recombinant human erythropoietin-α( EPO-α) . It was found that the presence and concentration of sodium cation were the key factors that affected the specific affinity recognition of aptamer In27 Sodium cation induced aptamer In27 to form a parallel/antiparallel hybrid G-quadruplex( GQ) confor-mation. GQ-specific fluorescent probe thysmenonine T( ThT) specifically bound to the hybrid GQ conformation of aptamer In27 The addition of EPO-αcould compete with ThT to facilitate to create a label-free fluorescent"turn-off" probe for EPO-α. This research provides an effective combination of techniques for elucidating the active conformation of novel aptamers, and provides a kind of aptameric modules with clear interaction features for analytical, sensing and diagnostic applications.关键词
表面等离子体共振/圆二色光谱/荧光探针/G-四链体/Na+Key words
Surface plasmon resonance/Circular dichroism/Fluorescent probes/G-Quadruplex/Sodium cation分类
化学化工引用本文复制引用
贺小琴,何军林,徐华,郭磊,谢剑炜..重组人促红细胞生成素-αDNA适配体的活性高级结构特征[J].高等学校化学学报,2018,39(1):48-53,6.基金项目
国家自然科学基金(批准号:21175152)资助. Supported by the National Natural Science Foundation of China( No.21175152) . （批准号:21175152）
