广东医学2017，Vol.38Issue(22)：3393-3396,4.
Fmr1基因敲除小鼠大脑的磁共振波谱研究
Brain magnetic resonance spectroscopy of Fmr1 knockout mice
摘要
Abstract
Objective To investigate the changes of metabolites in brain of Fmr 1 knockout mice ( KO mice) by 1H-MR spectroscopy (1H-MRS) and its application in diagnosis of Fragile X Syndrome (FXS).Methods 1H-MRS was performed on 23 KO mice (experimental group) and 16 wild type mice (control group) at the age of 4 months. The sub-peak areas of N-aeetyl aspartate (NAA), choline (Cho) and creatine (Cr) in the cerebral codex and hippo-campus were measured , and the NAA/Cr and Cho/Cr ratios were calculated and compared by two -sample t-test.Re-sults Compared with control group , the NAA/Cr ratio was significantly reduced (t=-5.912, P<0.01), and the Cho/Cr ratio was significantly increased ( t=3.888, P<0.01) in KO mice.Conclusion 1 H-MRS examination can quanti-tatively assess abnormal metabolites in the brains of KO mice , which may provide important clues for the diagnosis of FXS and evaluation of therapeutic efficacy .关键词
脆性X综合征/Fmr1基因敲除小鼠/磁共振波谱成像Key words
Fragile X Syndrome/Fmr1 knockout mice/Magnetic resonance spectroscopy引用本文复制引用
郭艺,陈盛强,黄勇,谭理连,彭土康,林秋喜..Fmr1基因敲除小鼠大脑的磁共振波谱研究[J].广东医学,2017,38(22):3393-3396,4.基金项目
国家自然科学基金资助项目(编号:31771327),广东省科技计划项目(编号:2015A0030302090),广州市医药卫生科技项目(编号:20141A011087,20151A011080) （编号:31771327）
