广东医学2017，Vol.38Issue(22)：3397-3400,4.
活化诱导胞嘧啶核苷脱氨酶在骨癌痛-吗啡耐受大鼠脊髓中的表达变化
The expression of activation induced cytidine deaminase in spinal cord of bone cancer pain-morphine tolerance rats.
摘要
Abstract
Objective To investigate the possible mechanism of bone cancer pain -morphine tolerance by evalu-ating spinal cord activation induced cytidine deaminase ( AI-CDA) in rats with bone cancer pain -morphine tolerance . Methods Thirty healthy female Wistar rats (weighing 160~180 g) were randomly divided into 3 groups (n=10), con-trol group ( Group Ⅰ) , bone cancer pain group ( Group Ⅱ) and bone cancer pain -morphine tolerance group ( GroupⅢ) .Nasopharyngeal carcinoma HONE 1 cells were inoculated in the left hind tibia bone marrow cavity for rat model of bone cancer pain.After operation, the withdraw mechanical threshold (MWT) was assessed every day, and the damage of bone was assessed with X -Ray on the 14 th day after operation .The model rats in Group Ⅲreceived subcutaneous injec-tion of morphine (10 mg/kg) from the 15th to the 21st day after operation, whereas equal dose of normal saline was applied as placebo in Group Ⅱ.The inoculated tibia and the spinal cord specimen (L4-6) were obtained on 21st day.The AI-CDA mRNA expression was assessed by RT -PCR.Results Compared with Group Ⅱ, during the first 3 days of mor-phine therapy (15th to 17th day), the MWT in Group Ⅲ was significantly reduced (P<0.05).The MWT in Group Ⅲduring the first 3 days of morphine therapy (15th to 17th day) was significantly higher than that during the last 3 days of morphine therapy (18th to 21st day) (P<0.05).Bone lesions and carcinoma cell inoculation were observed in both GroupⅡ and Ⅲaccording to radiography and pathological study , respectively .Compared with Group Ⅱ, the expression of AI-CDA mRNA was increased in Group Ⅲ ( P<0.05 ) .Conclusion The expression of AI -CDA is increased in spinal cord and may participate in development of bone cancer pain -morphine tolerance .关键词
骨癌痛/吗啡耐受/AI-CDA/脊髓Key words
bone cancer pain/morphine tolerance/AI-CDA/spinal cord引用本文复制引用
秦明秀,李昌龙,朱敏,侯伟楠,周艳琼,覃艳君,梁锐,袁斌毅..活化诱导胞嘧啶核苷脱氨酶在骨癌痛-吗啡耐受大鼠脊髓中的表达变化[J].广东医学,2017,38(22):3397-3400,4.基金项目
广西自然科学基金资助项目(编号:2011GXNSFA018193) （编号:2011GXNSFA018193）
