广东医学2017，Vol.38Issue(22)：3401-3404,4.
miR-34 a在缺氧复氧介导心肌细胞凋亡中的作用以及当归补血汤的干预效果
Study on the role of miR-34a in cardiomyocyte apoptosis induced by reoxygenation after hypoxia and the inter-vention of Danggui Buxue Decoction
摘要
Abstract
Objective To investigate the role of miR -34a in hypoxia/reoxygenation -mediated cardiomyocyte apoptosis and the effect of Danggui Buxue Decoction .Methods Cultured neonatal rat ventricular myocytes were prepared by hypoxia and reoxygenation model , and divided into control group , miR-34a inhibitory group and low -, medium-and high-dose Danggui Buxue Decoction groups .Cell counting kit-8 method was used to detect the viability of cardio-myocytes.Flow cytometry was used to detect the apoptosis of cardiomyocytes .The expression of miR -34a was assessed by quantitative PCR .The expression of Bcl -2 was assessed by Western blot .Results The apoptosis ratio in control group, low-, medium-and high-dose Danggui Buxue Decoction groups and miR -34a inhibitory group were (35.00 ± 4.69)%, (33.00 ±5.55)%, (28.83 ±4.49)%, (25.33 ±5.43)%and (23.17 ±5.04)%, respectively.In contrast to control group , the cell survive ratios in low-, medium-and high-dose Danggui Buxue Decoction groups and miR -34a inhibitory group were 1.290 ±0.130, 1.822 ±0.123, 2.082 ±0.149 and 2.238 ±0.247, respectively.In contrast to control group, the miR-34a expression in low -, medium -and high -dose Danggui Buxue Decoction groups and miR-34a inhibitory group were 0.877 ±0.071, 0.705 ±0.045, 0.580 ±0.077 and 0.453 ±0.076, respectively.Com-pared with control group , the viability of cardiomyocytes and the expression of Bcl -2 protein were significantly improved , and the expression of miR -34a was decreased in miR-34a inhibitory group and low-, medium-and high-dose Dan-ggui Buxue Decoction groups ( all P<0.05 ) .Compared with control group and low -dose Danggui Buxue Decoction group, the apoptosis of cardiomyocytes in medium -and high -dose Danggui Buxue Decoction group were decreased (P<0.05).Conclusion Danggui Buxue Decoction can effectively inhibit the apoptosis of cardiomyocytes , which is closely related to the inhibition of miR -34a expression.关键词
心肌细胞/细胞凋亡/当归补血汤/miR-34aKey words
cardiomyocytes/cell apoptosis/Danggui Buxue Decoction/miR-34a引用本文复制引用
范智文,赵一俏,凌龙,席玉胜,李铭,靳文,崔同涛,徐基瑛..miR-34 a在缺氧复氧介导心肌细胞凋亡中的作用以及当归补血汤的干预效果[J].广东医学,2017,38(22):3401-3404,4.基金项目
广东省自然科学基金资助项目(编号:2016A030313853),广东省中医药局科研课题(编号:20151041),中国人民解放军第四二一医院医学创新项目(编号:201604) （编号:2016A030313853）
