广东医学2017，Vol.38Issue(24)：3784-3787,3791,5.
亲环素D对氧化应激诱发的肾小管上皮细胞坏死的影响
Oxidative stress induces renal tubular epithelial cells necrosis in a cyclophilin D-Dependent Manner
摘要
Abstract
Objective To investigate the role of cyclophilin D ( Cyp D ) on oxidative stress -induced necrosis in renal tubular epithelial cells .Methods Eighteen male Wistar rats were randomly divided into Control group , Sham con-trol group and Ischemia reperfusion injury group .HE staining was carried out and serum creatinine was measured .H2 O2 was used to induce oxidative stress by different times or concentrations .Cyp D-siRNA or CsA were applied as pretreat-ment .The manner of cell death was identified by flow cytometry .Results Serum creatinine was significantly increased in Ischemia reperfusion injury group than those in Control group and Sham control group (P=0.017, P=0.018).Proximal renal tubulars expansion , loss of brush border , and a large number of tube type and cell vacuoles degeneration were ob-served in Ischemia reperfusion injury group by HE staining .H2 O2 induced HK-2 cells necrosis in dose -and time-de-pendent manners, which was inhibited by Cyp D -siRNA and CsA (P=0.026, P=0.008).Conclusion Cyp D plays an important role in oxidative stress -induced necrosis , which could be ameliorated by inhibiting Cyp D .关键词
亲环素D/肾小管上皮细胞/坏死/急性肾损伤/氧化应激Key words
cyclophilin D/renal tubular epithelial cell/necrosis/acute kidney injury/oxidative stress引用本文复制引用
杨欢,李锐钊,董伟,张舒,刘双信,梁馨苓..亲环素D对氧化应激诱发的肾小管上皮细胞坏死的影响[J].广东医学,2017,38(24):3784-3787,3791,5.基金项目
国家自然科学基金资助项目(编号:81570609,81400738),广东省自然科学基金资助项目(编号:2014A030313545,2015A030313531) （编号:81570609,81400738）
