国际妇产科学杂志2017，Vol.44Issue(6)：690-694,5.
应用飞行时间质谱分析卵巢癌相关肿瘤标记物
Application of Time-of-flight Mass Spectrometry for Serum Biomarkers in Ovarian Cancer
摘要
Abstract
Objective:To analyze the serum biomarkers of the ovarian cancer patients, to establish serum diagnosis models and to find potential biomarkers with MALDI-TOF-MS combined with WCX magnetic bead. Methods:8 healthy controls, 22 benign ovarian tumor, 16 borderline tumor, and 16 ovarian cancer patients were enrolled to pre-extract the serum samples using the MB-WCX kit. Then, Biomarker Wizard software was used to detect protein peaks and potential difference between these groups. Last comparing with CA125 in sensitivity and specificity. Results:A diagnostic pattern for borderline tumor from healthy controls using m/z 4508.71 was established with 100% sensitivity and 62.5% specificity, for borderline tumor from benign healthy controls was 83.3% sensitivity and 72.8% specificity, and m/z 2665.32 for ovarian cancer from borderline tumor was established with 100% sensitivity and 50.0% specificity. Further compared to traditional CA125 (sensitivity 76%), the sensitivity of this methods is higher (compared with normal control group was 100%, compared with benign tumor group of 83.3%). Conclusions:MALDI-TOF-MS can be used not only to establish serum diagnosis models, but also to search for potential biomarkers in ovarian cancer patients′serum samples.关键词
卵巢肿瘤/气相色谱-质谱法/肿瘤标记,生物学/基质辅助激光解吸离子飞行时间质谱Key words
Ovarian neoplasms/Gas chromatography-mass spectrometry/Tumor markers,biological/Matrix-assisted laser desorption ionization time-of-flight mass spectrometry引用本文复制引用
刘贵芬,宋一一,孙蓬明,阮冠宇..应用飞行时间质谱分析卵巢癌相关肿瘤标记物[J].国际妇产科学杂志,2017,44(6):690-694,5.基金项目
2014年福建省自然科学基金项目(2014J01278) （2014J01278）
2015年福建省卫生系统中青年骨干人才培养项目(2014-ZQN-ZD-5) （2014-ZQN-ZD-5）
