国际口腔医学杂志2018，Vol.45Issue(1)：26-31,6.DOI:10.7518/gjkq.2018.01.005
溶血磷脂酸对牙髓细胞中β-连环蛋白核转位的影响及机制初探
The effects and underlying mechanism of lysophosphatidic acid in β-catenin nuclear translocation of dental pulp cells
摘要
Abstract
Objective The aim of our study is to investigate the biological effects of lysophosphatidic acid (LPA) on β-catenin accumulation, activation, and nuclear translocation of cultured human dental pulp cells (DPCs) ex vivo.MethodsLPA was used to stimulate dental pulp cells, and Y-27632 was used to inhibit Rho-associated protein kinase (ROCK). Fluorescence microscopy and Western blot were used to detect the effects of Rho/ROCK on β-catenin accumulation, activation, and nuclear transloca-tion. Results β-catenin accumulated around the nuclear membrane after LPA stimulation of DPC for 3 h. When treated for 6 or 10 h with LPA, β-catenin translocated into the nucleus in some dental pulp cells; however, nuclear translocation of β-catenin was abrogated by Y-27632. Results of Western blot showed that LPA promoted β-catenin expression and activation. ROCK inhibition also degraded the level of β-catenin activation.Conclusion LPA regulated β-catenin accumulation, activation, and nuclear translocation via the Rho/ROCK signaling pathway.关键词
牙髓细胞/溶血磷脂酸/Rho相关蛋白激酶/β-连环蛋白Key words
dental pulp cell/lysophosphatidic acid/Rho-associated protein kinase/β-catenin分类
医药卫生引用本文复制引用
房宏志,杨惠,邵美瑛,胡涛..溶血磷脂酸对牙髓细胞中β-连环蛋白核转位的影响及机制初探[J].国际口腔医学杂志,2018,45(1):26-31,6.基金项目
国家自然科学基金(81400504) （81400504）
四川省科学技术厅应用基础研究项目(2014JY0073,2013JY0164) （2014JY0073,2013JY0164）
中国博士后科学基金面上项目(2014M562332)This study was supported by National Natural Science Foundation of China (81400504), Applied Basic Research Project of Sichuan Provincial Science and Technology Department (2014JY0073, 2013JY0164) and China Postdoctoral Science Foundation (2014M562332). （2014M562332）
