海南医学院学报2017，Vol.23Issue(23)：3187-3190,4.DOI:10.13210/j.cnki.jhmu.20171207.001
科罗索酸对妊娠期糖尿病大鼠胎盘组织中基因表达的调节作用及对胰岛β细胞的保护作用研究
The regulating effect of corosoic acid on gene expression in placental tissue of rats with ges-tational diabetes mellitus and its protective effect on islet β cells
摘要
Abstract
Objective:To study the regulating effect of corosoic acid on gene expression in placental tissue of rats with gestational diabetes mellitus and its protective effect on islet β cells. Methods:SD female rats were divided into corosoric acid group(CA group),gestational diabetes mellitus group(GDM group)and control group,CA group and GDM group were es-tablished into GDM models,and CA group were given corosoric acid intervention. At 20 days of gestation,the expression of insulin signal transducers and inflammatory reaction molecules in placenta and the expression of apoptosis molecules in islet were measured. Results:p-IRS1,p-IRS2,p-PI3K,GLUT1,GLUT3,GLUT4 and visfatin protein expression in placenta tis-sue as well as Bcl-2 mRNA expression in islet tissue of GDM group were significantly lower than those of control group where-as NF-kB,ICAM-1,TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression inislet tissue were significantly higher than those of control group ;p-IRS1 ,p-IRS2 ,p-PI3K ,GLUT1 ,GLUT3 ,GLUT4 and visfatin protein expression in placenta tissue as well as Bcl-2 mRNA expression in islet tissue of CA group were significantly higher than those of GDM group whereas NF-kB ,ICAM-1 ,TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression in islet tissue were significantly lower than those of GDM group. Corosoic acid can improve the insulin resistance and reduce the islet β cell injury in rats with gestational diabetes mellitus.关键词
妊娠期糖尿病/科罗索酸/胰岛素抵抗/细胞凋亡Key words
Gestational diabetes mellitus/Corosoic acid/Insulin resistance/Apoptosis分类
医药卫生引用本文复制引用
黄晓萍,张娟,王文平,任丹..科罗索酸对妊娠期糖尿病大鼠胎盘组织中基因表达的调节作用及对胰岛β细胞的保护作用研究[J].海南医学院学报,2017,23(23):3187-3190,4.基金项目
四川省高校人才培养计划项目2011021BThis study was supported by Talent Training Program for Universities in Sichuan(2011021B) （2011021B）
