海南医学院学报2017，Vol.23Issue(23)：3191-3194,3198,5.DOI:10.13210/j.cnki.jhmu.20171130.002
丹皮酚联合γ射线对脑胶质瘤细胞凋亡程度及凋亡基因表达的影响
Influence of paeonol combined with γ ray on glioma cell apoptosis and apoptosis gene ex-pressiona
摘要
Abstract
Objective:To study the influence of paeonol combined with γ ray on glioma cell apoptosis and apoptosis gene expression. Methods:Glioma C6 cells were cultured and divided into control group(treated with serum-free DMEM),ray group(exposed to 4Gy γ ray),Pae group(treated with serum-free DMEM contains 4μg/mL paeonol)and γ+ Pae group(trea-ted with serum-free DMEM contains 4μg/mL paeonol and exposed to 4Gy γ ray). The cell proliferation activity value as well as the expression of pro-apoptosis genes and anti-apoptosis genes was measured after the intervention. Results:After 6 hours,12 hours,18 hours and 24 hours of intervention,cell proliferation activity value of ray group,Pae group and γ+ Pae group weregreatly lower than that of control group ,and cell proliferation activity value of γ + Pae group was obviously lower than that of ray group and Pae group. After 24 hours of intervention ,p21cip1 ,TRAIL ,LRIG1 ,NPRL2 and SEPT7 mRNA expression in ray group ,Pae group and γ + Pae group were notably higher than those in control group whereas PIAS3 ,CEACAM1 ,EZH2 ,MDM2 ,c-myc and Survivin mRNA expression were greatly lower than those in control group ;p21cip1 , TRAIL ,LRIG1 ,NPRL2 and SEPT7 mRNA expression in γ + Pae group were remarkably higher than those in ray group and Pae group whereas PIAS3 ,CEACAM1 ,EZH2 ,MDM2 ,c-myc and Survivin mRNA expression were greatly lower than those in ray group and Pae group. Conclusion :Paeonol combined with γ ray can be more effective than γ ray alone in inducing the apoptosis of glioma cells ,increasing the expression of pro-apoptosis genes and decreasing the expression of anti-apoptosis genes.关键词
胶质瘤/放疗/丹皮酚/γ射线/凋亡Key words
glioma/radiotherapy/paeonol/γ ray/apoptosis分类
医药卫生引用本文复制引用
任正婷,张帆..丹皮酚联合γ射线对脑胶质瘤细胞凋亡程度及凋亡基因表达的影响[J].海南医学院学报,2017,23(23):3191-3194,3198,5.基金项目
安徽省自然科学基金(1308085M H134)This study was supported by Anhui Natural Science Foundation(Grant No. 1308085MH134) （1308085M H134）
