海南医学院学报2017，Vol.23Issue(23)：3243-3246,4.DOI:10.13210/j.cnki.jhmu.20171127.003
MEST对子痫前期胎盘中新生血管形成、滋养细胞侵袭的影响
Effect of MEST on the angiogenesis and trophoblast cell invasion in the preeclampsia pla-centa
摘要
Abstract
Objective:To study the effect of mesoderm-specific transcript(MEST)on the angiogenesis and trophoblast cell invasion in the preeclampsia placenta.Methods:The puerperae who were diagnosed with preeclampsia and gave birth in our hospital between June 2014 and February 2017 were selected as the PE group,and the healthy puerperae who gave birth in our hospital during the same period were selected as the control group.After delivery,the placental tissue was collected,fluores-cence quantitative PCR method was used to determine MEST mRNA expression,and enzyme-linked immunosorbent assay was used to determine the protein levels of angiogenesis molecules and cell invasion molecules.Results:MEST,Notch1,DLL4, VEGF,Rho,ROCK,LAMA4,Notch2 and Notch3 protein levels in placental tissue of PE group were obviously lower than those of control group whereas DNC,sFlt-1 and sEng protein levels were obviously higher than those of control group;Notch1,DLL4,VEGF,Rho,ROCK,LAMA4,Notch2 and Notch3 protein levels in PE placenta tissue with lower MEST ex-pression were greatly lower than those in PE placenta tissue with higher MEST expression whereas DNC,sFlt-1 and sEng pro-tein levels were greatly higher than those in PE placenta tissue with higher MEST expression.Conclusions:The low expression of MEST in preeclampsia placenta can inhibit the angiogenesis and trophoblast cell invasion.关键词
子痫前期/中胚层特异性转录基因/血管新生/细胞侵袭Key words
Preeclampsia/Mesoderm-specific transcript/Angiogenesis/Cell invasion分类
医药卫生引用本文复制引用
张秀琼,刘玉琴..MEST对子痫前期胎盘中新生血管形成、滋养细胞侵袭的影响[J].海南医学院学报,2017,23(23):3243-3246,4.基金项目
自贡市科技局课题项目(J2015036)This project is funded by Research Project of Science and Technology Bureau of Zigong City(J2015036) （J2015036）
