解放军医药杂志2017，Vol.29Issue(12)：10-12,24,4.DOI:10.3969/j.issn.2095-140X.2017.12.003
miR-29c靶向调控AMPK/mTOR信号通路对胃癌细胞生物学行为的影响
Effect of miR-29c Targeting on AMPK/mTOR Signaling Pathway on Biological Behavior of Gastric Cancer Cells
摘要
Abstract
Objective To investigate affecting mechanism of miR-29c targeting AMPK/mTOR signaling pathway on biological behavior of gastric cancer cells. Methods The miR-29c expressions in different gastric cancer cell lines were detected by quantitative polumerase chain reaction ( qPCR) . Immunofluorescence was used to detect transfection efficiency of miR-29c-mimic transfecting SGC-7901 gastric cancer cell. MTT proliferation assay was used to detect effect of miR-29c on proliferation ability of SGC-7901 gastric cancer cell. Transwell invasive test was used to detect effect of miR-29c on inva-sion ability of SGC-7901 gastric cancer cell. Western blotting was used to detect changes of p-AMPK and PRR5 protein ex-pressions after miR-29c overexpression. Results The miR-29c expression in SGC-7901 gastric cancer cell was significantly lower than those in MGC-803, AGS and HGC-27 gastric cancer cells (P<0. 05). At 72 h after transfection, miR-29c-mimic could effectively transfect into SGC-7901 gastric cancer cell. Values of cell proliferation rate and expression levels of p-AMPK and PRR5 proteins in miR-29c-mimic group were significantly lower than those in NC group (P<0. 05), while number of cells migrated to the submucosa was significantly less than that in NC group (P<0. 05). Conclusion miR-29c may target AMPK/mTOR signaling pathway to regulate biological behavior of gastric cancer cells.关键词
胃肿瘤/微RNA-29c/细胞增殖/侵袭Key words
Stomach neoplasms/MicroRNA-29c/Cell proliferation/Invasiveness分类
医药卫生引用本文复制引用
魏耕富,王健生,马智,权力,智明,周明亮,周杰,刘红冬..miR-29c靶向调控AMPK/mTOR信号通路对胃癌细胞生物学行为的影响[J].解放军医药杂志,2017,29(12):10-12,24,4.基金项目
陕西省科学技术研究发展计划项目(2014KW23-02) （2014KW23-02）
