河南农业大学学报2017，Vol.51Issue(6)：801-807,7.
萼脊兰AGAMOUS(AG)基因的克隆与表达分析
Analysis of cloning and expression analysis of AGAMOUS gene from Sedirea japonica
摘要
Abstract
The structure,function,clone of AGAMOUS(AG) gene as well as its expression regulation were studied in this paper.It's found that the amino acid sequence of AG belonged to plant type peculiar MIKC,C gene of MADS-box class. The full length cDNA of AG was 1 002 bp with an ORF of 702 bp encoding 233 putative amino acid residues(GenBank:KY744276). Multiple sequence alignments and phylogenetic tree analyses showed that AG was close to the phalaenopsis amabilis with an overall se-quence similarity. The encoded protein was hydrophilic and contained 48.93% of α-helical domains, 11.59% of extended strand,39.48% of random coil. The highest expression of the gene analyzed by real-time fluorescent quantitative PCR was in bud and stigma. It indicated that AG gene expression was tissue specificity,belonged to the class C gene characteristics in ABCDE model,and controlled the de-velopment of stamen and pistil.关键词
萼脊兰/AG基因克隆/RACE技术/实时荧光定量PCR/表达分析Key words
Sedirea japonica/AG gene cloning/RACE/real-time fluorescentquantitative PCR/gene expression分类
生物科学引用本文复制引用
蒋素华,邓祖丽颖,郝平安,王默霏,袁秀云,崔波..萼脊兰AGAMOUS(AG)基因的克隆与表达分析[J].河南农业大学学报,2017,51(6):801-807,7.基金项目
河南省高等学校重点科研项目(14B180036) （14B180036）
郑州市科技发展计划项目(20150448) （20150448）
郑州市普通科技攻关项目(141PPTGG420) （141PPTGG420）
