湖南大学学报（自然科学版）2017，Vol.44Issue(12)：127-134,8.DOI:10.16339/j.cnki.hdxbzkb.2017.12.020
液态培养条件下烟曲霉去除Cd的研究
Study on Removal of Cd by A s per gillus fum igatus in Liquid Culture
摘要
Abstract
The removal efficiency of a Cd-resistant Aspergillus fumigatus under different concentrations of Cd in laboratory flask culture was studied ,and the changes of glutathione (GSH) produc-tion in the strain were determined .The results showed that the removal rate of Cd was 55 .5％ when the concentration of Cd was 50 mg /L ,and the concentration of GSH was 0 .89 μmol/mg .The comparison of Cd removal efficiency by Aspergillus fumigatus under different conditions showed that the optimal condition was 30 ℃ ,pH value of 5 .0 ,add fluid volume of 120 mL ,speed of 100 r/min ,and inoculation amount of 0 .4 mL (1 .0 × 107 CFU /mL) .Under the optimal condition ,the removal rate of Cd was 29 .8％ higher than that under the initial condition ,and the GSH content in the culture system was increased by 0 .42 μmol/mg .The changes of glutathione peroxidase (GPX) and glutathione reductase (GR) indicated that Aspergillus fu-migatus could remove the heavy metal Cd in the culture system under the optimized liquid grow th condi-tions ,GSH could promote the removal of cadmium ,and its detoxification mechanism could be achieved by removing the oxidative substances produced by stress by GPX and GR in vivo .关键词
烟曲霉/谷胱甘肽/液体培养/镉/去除Key words
A spergillus fumigatus/glutathione/liquid culture/cadmium/removal分类
资源环境引用本文复制引用
陈耀宁,汪元南,黎媛萍,陈艳容,苟宇,朱福造,张道利,刘耀..液态培养条件下烟曲霉去除Cd的研究[J].湖南大学学报（自然科学版）,2017,44(12):127-134,8.基金项目
国家自然科学基金资助项目 (51378190 ,21276069 ,51579094 ) ,National Natural Science Foundation of China (51378190 , 21276069 ,51579094) （51378190 ,21276069 ,51579094 ）
