中国实用神经疾病杂志2017，Vol.20Issue(21)：12-15,4.DOI:10.3969/j.issn.1673-5110.2017.21.003
帕瑞昔布对脑缺血损伤大鼠神经保护作用的研究
Study on the neuroprotective effects of parecoxib on cerebral ischemia injury rats
摘要
Abstract
Objective To evaluate the Role of mitochondrial ATP-sensitive potassium channels in attenuation of cerebral is-chemia-reperfusion injury by parecoxib in rats .Methods One hundred healthy male Sprague-Dawley rats ,weighing 280g-330g , were randomly assigned into 5 groups (n=20 each) using a random number table:sham operation group (group S);focal cerebral I/R group (group I/R);parecoxib group (group P);5-hydroxydecanoate group (group 5-HD);5-hydroxydecanoate group+ pare-coxib group (group 5-HD+ P) .Focal cerebral ischemia was produced by 2h right middle cerebral artery occlusion followed by reperfusion .Group P received parecoxib 4mg/kg intravenously 15min before ischemia and again at 12h during reperfusion ,while group S and I/R received the equal volume of normal saline instead .In group 5-HD ,5-HD 30 mg/kg was injected intravenously at 1h before ischemia .In group 5-HD+P ,5-HD 30 mg/kg was injected intravenously at 1h before ischemia and the other procedures were similar to those previously described in group P .The neurological function deficit score (NDS) was evaluated in the 5 groups in 24h after reperfusion .Then the rats were sacrificed ,and their brains were immediately removed for evaluation of the levels of su-per oxide dismutase (SOD) ,malondialdehyde (MDA) ,Bax protein and Bcl-2 protein .Results Compared with group S ,the NDS , the levels of MDA and Bax protein increased ,and the levels of SOD and Bxl-2 protein decreased in the other groups (P<0.05) . Compared with group I/R ,the NDS ,the levels of MDA and Bax protein decreased ,and the levels of SOD and Bxl-2 protein in-creased in group P (P<0.05) .Compared with group P ,the NDS ,the levels of MDA and Bax protein increased ,and the levels of SOD and Bxl-2 protein decreased in 5-HD+ P (P<0.05) .Conclusion MitoKATP channels are involved in reduction of I/R-in-duced cerebral injury by parecoxib in rats .关键词
线粒体ATP敏感性钾离子通道/帕瑞昔布/脑缺血再灌注/氧化应激/细胞凋亡/大鼠Key words
Mitochondrial KATP channel/Parecoxib/Cerebral ischemia-reperfusion/Oxidative stress/Apoptosis/Rat分类
医药卫生引用本文复制引用
袁峰,付红光,孙凯,董铁立,杨鹏举..帕瑞昔布对脑缺血损伤大鼠神经保护作用的研究[J].中国实用神经疾病杂志,2017,20(21):12-15,4.基金项目
河南省基础与前沿技术研究计划项目(122300410403) （122300410403）
