中国实用神经疾病杂志2017，Vol.20Issue(24)：7-12,6.DOI:10.3969/j.issn.1673-5110.2017.24.002
趋化因子CC配基2对 α-Synuclein诱导的小胶质细胞增殖及神经元凋亡的影响
Study on the effect of CCL2 on the proliferation of microglia and apoptosis of Neuron induced by α-Synuclein
摘要
Abstract
Objective To investigate the effects of CCL2 on the proliferation of microglia and apoptosis of Neuron induced by α-Synuclein .Methods Primary microglia and primary neurons were isolated and cultured in vitro .The microglia were divided into 4 groups ,which were the control group ,the CCL2 group ,theα-Synuclein group ,the CCL2+ α-Synuclein group .In the control group ,the amount of PBS was added ,and the cells in the CCL2 group were added with the culture solution containing 0.05ng/μL concentration of CCL2.α-Synuclein group cells were added to culture medium containing a 0.2ng/μL concentration of α-Synuclein . CCL2+ α-Synuclein group were added with CCL20.05ng/μL ,0.2ng/μL α-Synuclein .After 24h culture ,the proliferation of micro-glia and the level of α-Synuclein ,the contents of TNF-α,IL-1βand NO in cell culture medium were detected .The primary neurons were cultured with cell culture medium in each group ,the neuron apoptosis was observed and the levels of Cleaved Caspase-3 Akt , p-Akt and were observed .Results The proliferation activity and secretion of TNF-α,IL-1β,NO in the CCL2 group ,the α-Synuclein group ,the CCL2+ α-Synuclein group were significantly higher than that in control group (P<0.05) .The level of α-Sy-nuclein in CCL2 group and CCL2+ α-Synuclein group was significantly higher than that in control group (P<0.01) .The apopto-sis rate and Cleaved Caspase-3 protein level of CCL2 group ,α-Synuclein group and CCL2+ α-Synuclein group were significantly higher than those in control group ,the level of p-Akt was lower than that of control group (P<0.01) .Conclusion CCL2 pro-motes the proliferation of the microglia and secretion of TNF-α,IL-1β,NO induced by α-Synuclein ,and can promotes the apoptosis of neuron ,the mechanism may be related to Akt signaling pathway .关键词
小胶质细胞/神经元/CCL2/凋亡/信号通路/小鼠Key words
Microglia/Neuron/CCL2/Apoptosis/Signal pathway/Rat分类
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张黎军,赵盼盼,赵建华,王超伟,李青,随瑞斌,袁彬..趋化因子CC配基2对 α-Synuclein诱导的小胶质细胞增殖及神经元凋亡的影响[J].中国实用神经疾病杂志,2017,20(24):7-12,6.基金项目
河南省科技厅科技攻关项目 ,编号 :172102310685 （）
